Takemura N, Koyama H, Sako T, Ando K, Suzuki K, Motoyoshi S, Marumo F
Department of Veterinary Internal Medicine, Nippon Veterinary and Zootechnical College, Tokyo, Japan.
Res Vet Sci. 1991 Jan;50(1):86-8. doi: 10.1016/0034-5288(91)90058-v.
The concentration and molecular form of the plasma atrial natriuretic peptide (ANP) in dogs with mitral regurgitation was investigated. Plasma ANP concentration in dogs with mitral regurgitation was significantly increased (29.4 +/- 1.88 pmol litre-1, n = 40) compared to that in the controls (14.5 +/- 0.62 pmol litre-1, n = 20, P less than 0.01). Molecular forms of plasma ANP were determined by the gel permeation chromatogram. A single peak corresponding to alpha-ANP was detected in the plasma from the controls. However, a peak corresponding to beta-ANP and, or, gamma-ANP was detected in the plasma of the dogs with mitral regurgitation in addition to alpha-ANP. These results suggest that the process of ANP synthesis was altered and excretion of ANP from the heart was enhanced in dogs with mitral regurgitation.
研究了二尖瓣反流犬血浆心钠素（ANP）的浓度和分子形式。与对照组相比，二尖瓣反流犬的血浆ANP浓度显著升高（29.4±1.88 pmol/L，n = 40），对照组为（14.5±0.62 pmol/L，n = 20，P＜0.01）。通过凝胶渗透色谱法测定血浆ANP的分子形式。在对照组血浆中检测到一个对应于α-ANP的单峰。然而，除α-ANP外，在二尖瓣反流犬的血浆中还检测到一个对应于β-ANP和/或γ-ANP的峰。这些结果表明，二尖瓣反流犬的心钠素合成过程发生改变，心脏心钠素排泄增强。
