Nayidu Naghabushana K, Wang Lei, Xie Weibo, Zhang Chengjun, Fan Chuanzhu, Lian Xingming, Zhang Qifa, Xiong Lizhong
National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research Wuhan, Huazhong Agricultural University, Wuhan 430070, China.
Gene. 2008 Apr 15;412(1-2):59-70. doi: 10.1016/j.gene.2008.01.006. Epub 2008 Jan 26.
PEX11 gene family has been shown to be involved in peroxisome biogenesis but very little is known about this gene family in rice. Here we show that five putative PEX11 genes (OsPEX11-1-5) present in rice genome and each contain three conserved motifs. The PEX11 sequences from rice and other species can be classified into three major groups. Among the five rice PEX11 genes, OsPEX11-2 and -3 are most likely duplicated. Expression profile and RT-PCR analysis suggested that the members of PEX11 family in rice had differential expression patterns: OsPEX11-1 and OsPEX11-4 had higher expression levels in leaf tissues than in the other tissues, OsPEX11-2 was detected only in germinated seeds, OsPEX11-3 was expressed predominantly in endosperm and germinated seeds, and OsPEX11-5 was expressed in all the tissues investigated. We also observed that the rice PEX11 genes had differential expression patterns under different abiotic stresses. OsPEX11-1 and OsPEX11-4 were induced by abscisic acid (ABA), hydrogen peroxide (H2O2), salt and low nitrogen stress conditions. OsPEX11-3 was responsive to ABA and H2O2 treatments, and OsPEX11-5 was responsive to ABA, H2O2, and salt treatments. However, OsPEX11-2 had no response to any of the stresses. Our results suggest that the rice PEX11 genes have diversification not only in sequences but also in expression patterns under normal and various stress conditions.
PEX11基因家族已被证明参与过氧化物酶体生物发生，但在水稻中对该基因家族的了解却非常少。在此我们表明，水稻基因组中存在五个假定的PEX11基因（OsPEX11 - 1 - 5），每个基因都包含三个保守基序。水稻和其他物种的PEX11序列可分为三大类。在五个水稻PEX11基因中，OsPEX11 - 2和 - 3很可能是重复基因。表达谱和逆转录 - 聚合酶链反应分析表明，水稻中PEX11家族成员具有不同的表达模式：OsPEX11 - 1和OsPEX11 - 4在叶片组织中的表达水平高于其他组织，OsPEX11 - 2仅在萌发种子中被检测到，OsPEX11 - 3主要在胚乳和萌发种子中表达，而OsPEX11 - 5在所有研究的组织中均有表达。我们还观察到，水稻PEX11基因在不同非生物胁迫下具有不同的表达模式。OsPEX11 - 1和OsPEX11 - 4在脱落酸（ABA）、过氧化氢（H2O2）、盐和低氮胁迫条件下被诱导表达。OsPEX11 - 3对ABA和H2O2处理有响应，而OsPEX11 - 5对ABA、H2O2和盐处理有响应。然而，OsPEX11 - 2对任何胁迫均无响应。我们的结果表明，水稻PEX11基因不仅在序列上，而且在正常和各种胁迫条件下的表达模式上都具有多样性。
