Huang Jianyan, Zhao Xiaobo, Yu Huihui, Ouyang Yidan, Wang Lei, Zhang Qifa
National Key Laboratory of Crop Genetic Improvement, National Centre of Plant Gene Research (Wuhan), Huazhong Agricultural University, 430070 Wuhan, China.
Plant Mol Biol. 2009 Oct;71(3):207-26. doi: 10.1007/s11103-009-9518-6. Epub 2009 Jul 16.
Ankyrin repeat (ANK) containing proteins comprise a large protein family. Although many members of this family have been implicated in plant growth, development and signal transduction, only a few ANK genes have been reported in rice. In this study, we analyzed the structures, phylogenetic relationship, genome localizations and expression profiles of 175 ankyrin repeat genes identified in rice (OsANK). Domain composition analysis suggested OsANK proteins can be classified into ten subfamilies. Chromosomal localizations of OsANK genes indicated nine segmental duplication events involving 17 genes and 65 OsANK genes were involved in tandem duplications. The expression profiles of 158 OsANK genes were analyzed in 24 tissues covering the whole life cycle of two rice genotypes, Minghui 63 and Zhenshan 97. Sixteen genes showed preferential expression in given tissues compared to all the other tissues in Minghui 63 and Zhenshan 97. Nine genes were preferentially expressed in stamen of 1 day before flowering, suggesting that these genes may play important roles in pollination and fertilization. Expression data of OsANK genes were also obtained with tissues of seedlings subjected to three phytohormone (NAA, GA3 and KT) and light/dark treatments. Eighteen genes showed differential expression with at least one phytohormone treatment while under light/dark treatments, 13 OsANK genes showed differential expression. Our data provided a very useful reference for cloning and functional analysis of members of this gene family in rice.
含有锚蛋白重复序列（ANK）的蛋白质构成了一个庞大的蛋白质家族。尽管该家族的许多成员都与植物的生长、发育和信号转导有关，但在水稻中仅报道了少数ANK基因。在本研究中，我们分析了在水稻中鉴定出的175个锚蛋白重复序列基因（OsANK）的结构、系统发育关系、基因组定位和表达谱。结构域组成分析表明，OsANK蛋白可分为十个亚家族。OsANK基因的染色体定位表明，涉及17个基因的9个片段重复事件以及65个OsANK基因参与了串联重复。在两个水稻基因型明恢63和珍汕97的整个生命周期的24个组织中，分析了158个OsANK基因的表达谱。与明恢63和珍汕97的所有其他组织相比，有16个基因在特定组织中表现出优先表达。9个基因在开花前1天的雄蕊中优先表达，表明这些基因可能在授粉和受精中发挥重要作用。还获得了用三种植物激素（萘乙酸、赤霉素3和激动素）处理的幼苗组织以及光/暗处理下OsANK基因的表达数据。18个基因在至少一种植物激素处理下表现出差异表达，而在光/暗处理下，13个OsANK基因表现出差异表达。我们的数据为水稻中该基因家族成员的克隆和功能分析提供了非常有用的参考。
