Dai Xiaoxia, You Changjun, Wang Lei, Chen Guoxing, Zhang Qifa, Wu Changyin
National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, 430070 Wuhan, China.
Plant Mol Biol. 2009 Nov;71(4-5):469-81. doi: 10.1007/s11103-009-9537-3. Epub 2009 Aug 18.
COBRA-like proteins play important roles in cell expansion and cell wall biosynthesis in Arabidopsis. In rice, a COBRA-like gene, BRITTLE CULM1 (BC1), has been identified as a regulator controlling the culm mechanical strength. Analysis of the rice genome indicated that BC1 belongs to an 11-member multigene family, termed the OsBC1L family in this study. Based on sequence comparisons and phylogenetic analysis, the OsBC1L family comprises two main subgroups. Expression patterns examined by microarray and reverse transcription polymerase chain reaction revealed that OsBC1L genes exhibit universal or specific expression patterns. Through T-DNA or Tos17 insertion mutant lines, the functions of six OsBC1L family members have been examined by investigating the phenotype variations of knockout mutants under normal growth conditions. Results suggest that the OsBC1L genes perform a range of functions and participate in various developmental processes in rice.
类COBRA蛋白在拟南芥的细胞扩张和细胞壁生物合成中发挥重要作用。在水稻中，一个类COBRA基因，脆茎1（BC1），已被鉴定为控制茎秆机械强度的调控因子。水稻基因组分析表明，BC1属于一个由11个成员组成的多基因家族，本研究中将其命名为OsBC1L家族。基于序列比较和系统发育分析，OsBC1L家族包括两个主要亚组。通过微阵列和逆转录聚合酶链反应检测的表达模式表明，OsBC1L基因呈现普遍或特异性的表达模式。通过T-DNA或Tos17插入突变体系，通过研究正常生长条件下敲除突变体的表型变化，对六个OsBC1L家族成员的功能进行了研究。结果表明，OsBC1L基因具有一系列功能，并参与水稻的各种发育过程。
