Oliveira J M, Costa S A, Leonor I B, Malafaya P B, Mano J F, Reis R L
Department of Polymer Engineering, 3B's Research Group-Biomaterials, Biodegradables and Biomimetics, University of Minho, Braga, Portugal.
J Biomed Mater Res A. 2009 Feb;88(2):470-80. doi: 10.1002/jbm.a.31817.
A developmental composite scaffold for bone tissue engineering applications composed of hydroxyapatite (HA) and carboxymethylchitosan (CMC) was obtained using a coprecipitation method, which is based on the "autocatalytic" electroless deposition route. The results revealed that the pores of the scaffold were regular, interconnected, and possess a size in the range of 20-500 microm. Furthermore, the Fourier transform infra-red spectrum of the composite scaffolds exhibited all the characteristic peaks of apatite, and the appearance of typical bands from CMC, thus showing that coprecipitation of both organic and inorganic phases was effective. The X-ray diffraction pattern of composite scaffolds demonstrated that calcium-phosphates consisted of crystalline HA. From microcomputed tomography analysis, it was possible to determine that composite scaffolds possess a 58.9% +/- 6% of porosity. The 2D morphometric analysis demonstrated that on average the scaffolds consisted of 24% HA and 76% CMC. The mechanical properties were assessed using compressive tests, both in dry and wet states. Additionally, in vitro tests were carried out to evaluate the water-uptake capability, weight loss, and bioactive behavior of the composite scaffolds. The novel hydroxyapatite/carboxymethylchitosan composite scaffolds showed promise whenever degradability and bioactivity are simultaneously desired, as in the case of bone tissue-engineering scaffolding applications.
采用基于“自催化”化学沉积路线的共沉淀法，制备了一种用于骨组织工程应用的由羟基磷灰石（HA）和羧甲基壳聚糖（CMC）组成的发育复合支架。结果表明，该支架的孔隙规则、相互连通，尺寸范围为20 - 500微米。此外，复合支架的傅里叶变换红外光谱显示出磷灰石的所有特征峰以及CMC的典型谱带，表明有机相和无机相的共沉淀是有效的。复合支架的X射线衍射图谱表明，磷酸钙由结晶HA组成。通过微计算机断层扫描分析，可以确定复合支架的孔隙率为58.9%±6%。二维形态分析表明，支架平均由24%的HA和76%的CMC组成。使用压缩试验评估了其在干燥和潮湿状态下的力学性能。此外，还进行了体外试验，以评估复合支架的吸水能力、重量损失和生物活性行为。新型羟基磷灰石/羧甲基壳聚糖复合支架在同时需要可降解性和生物活性的情况下，如骨组织工程支架应用中，显示出了应用前景。
