Lee Chung-Sung, Hwang Hee Sook, Kim Soyon, Fan Jiabing, Aghaloo Tara, Lee Min
Division of Advanced Prosthodontics, University of California Los Angeles, CA 90095, USA.
Division of Diagnostic and Surgical Sciences, University of California Los Angeles, CA 90095, USA.
Adv Funct Mater. 2020 Oct 22;30(43). doi: 10.1002/adfm.202003717. Epub 2020 Sep 2.
Bone repair is a complex process involving the sophisticated interplay of osteogenic stem cells, extracellular matrix, and osteoinductive factors, and it is affected by bacterial toxins and oxidative stress. Inspired by the nature of plant-derived phytochemicals and inorganic-organic analogues of the bone extracellular matrix, we report herein the facile design of a nanoclay-organic hydrogel bone sealant (NoBS) that integrates multiple physico-chemical cues for bone regeneration into a single system. Assembly of phytochemical-modified organic chitosan and silica-rich inorganic nanoclay serves as highly biocompatible and osteoconductive extracellular matrix mimics. The decorated phytochemical exerts inherent bactericidal and antioxidant activities, and acts as an intermolecular networking precursor for gelation with injectable and self-healing capabilities. Moreover, the NoBS exerts osteoinductive effects mediated by the nanoclay, which regulates the Wnt/β-catenin pathway, along with the addition of osteoinductive signals, resulting in bone regeneration in a non-healing cranial defect. Engineering of this integrated bone graft substitute with multifunctional properties inspired by natural materials may suggest a promising and effective approach for creating a favorable microenvironment for optimal bone healing.
骨修复是一个复杂的过程，涉及成骨干细胞、细胞外基质和骨诱导因子之间复杂的相互作用，并且会受到细菌毒素和氧化应激的影响。受植物源植物化学物质和骨细胞外基质的无机-有机类似物特性的启发，我们在此报告了一种纳米粘土-有机水凝胶骨密封剂（NoBS）的简便设计，该密封剂将多种促进骨再生的物理化学线索整合到一个单一系统中。植物化学修饰的有机壳聚糖和富含二氧化硅的无机纳米粘土的组装充当了具有高度生物相容性和骨传导性的细胞外基质模拟物。修饰的植物化学物质具有固有的杀菌和抗氧化活性，并作为具有可注射和自愈能力的凝胶化分子间网络前体。此外，NoBS发挥由纳米粘土介导的骨诱导作用，纳米粘土调节Wnt/β-连环蛋白通路，并添加骨诱导信号，从而在不愈合的颅骨缺损中实现骨再生。受天然材料启发设计的这种具有多功能特性的集成骨移植替代物，可能为创造有利于最佳骨愈合的微环境提供一种有前景且有效的方法。
