Shinagawa K, Sato K, Konuma H, Matsusaka N, Sugii S
Department of Veterinary Public Health, Faculty of Agriculture, Iwate University, Japan.
J Vet Med Sci. 1991 Apr;53(2):167-71. doi: 10.1292/jvms.53.167.
Partially purified vascular permeability (VP) factor (VPF) of Bacillus cereus induced fluid accumulation in the ligated intestinal loops of mouse (MIL) and rabbit (RIL), suggesting that the VP activity may correlate with fluid accumulation in ligated intestinal loops of these animals. Fluid accumulation was observed at 6-8 hr in 55-67% of mouse intestinal loops inoculated with 40-50 immunodiffusion units (IDU) of partially purified VPF, whereas it was found at 2 hr in all loops with 400-600 IDU of partially purified VPF. In rabbit intestinal loops with 120-190 IDU of partially purified VPF, fluid accumulation was observed at 6 hr. From these findings, VPF produced by B. cereus can be easily detected in both MIL and RIL. The intestinal tissue of mouse intestinal loops was histopathologically damaged at different concentrations of the VPF to induce fluid accumulation. With 50 IDU of partially purified VPF, severe edema was found in the laminia proprial layer and submucosa. With 600 IDU of partially purified VPF, on the other hand, severe necrosis in the surface epithelium of villus and laminia proprial layer, and hyperemia in the submucosa were observed, suggesting that partially purified VPF may be cytotoxic and/or intestinecrotic.
蜡样芽孢杆菌的部分纯化血管通透性（VP）因子（VPF）可诱导小鼠结扎肠袢（MIL）和兔结扎肠袢（RIL）出现液体蓄积，这表明VP活性可能与这些动物结扎肠袢中的液体蓄积相关。在接种40 - 50免疫扩散单位（IDU）部分纯化VPF的小鼠肠袢中，55% - 67%在6 - 8小时观察到液体蓄积，而在接种400 - 600 IDU部分纯化VPF的所有肠袢中，2小时即发现液体蓄积。在接种120 - 190 IDU部分纯化VPF的兔肠袢中，6小时观察到液体蓄积。根据这些发现，蜡样芽孢杆菌产生的VPF在MIL和RIL中都能很容易地检测到。在不同浓度的VPF诱导液体蓄积时，小鼠肠袢的肠组织出现组织病理学损伤。50 IDU部分纯化VPF时，固有层和黏膜下层出现严重水肿。另一方面，600 IDU部分纯化VPF时，观察到绒毛表面上皮和固有层严重坏死，黏膜下层充血，这表明部分纯化的VPF可能具有细胞毒性和/或肠坏死性。
