Sarecka Beata, Zak Iwona, Krauze Jolanta
Department of Biochemistry and Medical Genetics, Medical University of Silesia, Medykow Str 18, 40-752 Katowice, Poland.
Clin Biochem. 2008 May;41(7-8):467-73. doi: 10.1016/j.clinbiochem.2008.01.028. Epub 2008 Feb 15.
To assess the relationship between IL-6 and PAI-1 polymorphisms and coronary artery disease (CAD) and to observe the interactions between these polymorphic variants and smoking in the CAD risk.
The study population consisted of 178 patients with angiographically documented CAD and 202 blood donors. The analyses of genetic polymorphisms were performed using the PCR-RFLP method.
The frequency of PAI-1 5G allele was higher in the entire CAD group than in control group (p=0.04, OR=1.35). Also the 5G allele carriers (4G5G+5G5G) were more frequent in patients than in controls (p=0.03, OR=1.93). The number of women carrying 5G allele was again significantly higher among patients (OR=10.95 p=0.0075). The IL-6 C allele frequency was higher only in the CAD male subgroup (p=0.035, OR=1.44). We found synergistic and cumulative effects between specific genotype patterns and smoking in determining the risk of CAD, especially between PAI-1(4G5G+5G5G)+IL-6(CC) and smoking (SIM=4.18 and p=0.0005, OR=9.20, respectively).
There are synergistic and cumulative effects of 5G allele of PAI-1 polymorphism and C allele of IL-6 polymorphism with smoking in determining their associated risk with CAD.
评估白细胞介素-6（IL-6）和纤溶酶原激活物抑制剂-1（PAI-1）基因多态性与冠状动脉疾病（CAD）之间的关系，并观察这些多态性变体与吸烟在CAD风险中的相互作用。
研究人群包括178例经血管造影证实患有CAD的患者和202名献血者。采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法进行基因多态性分析。
整个CAD组中PAI-1 5G等位基因的频率高于对照组（p = 0.04，比值比[OR]=1.35）。患者中5G等位基因携带者（4G5G + 5G5G）也比对照组更常见（p = 0.03，OR = 1.93）。携带5G等位基因的女性患者数量再次显著高于对照组（OR = 10.95，p = 0.0075）。IL-6 C等位基因频率仅在CAD男性亚组中较高（p = 0.035，OR = 1.44）。我们发现特定基因型模式与吸烟之间在确定CAD风险方面存在协同和累积效应，特别是PAI-1(4G5G + 5G5G)+IL-6(CC)与吸烟之间（协同指数[SIM]=4.18，p = 0.0005，OR分别为9.20）。
PAI-1基因多态性的5G等位基因和IL-6基因多态性的C等位基因与吸烟在确定它们与CAD相关风险方面存在协同和累积效应。
