Gismondo M R, Chisari G, Lo Bue A M
Institute of Microbiology, University of Catania, Italy.
Drugs Exp Clin Res. 1991;17(2):101-4.
The general antibacterial properties of trospectomycin (TRO) were compared with those of spectinomycin (SPEC) in an in vitro study using a collection of recent clinical isolates: 50 Gram-positive and 25 Gram-negative aerobes and 30 Gram-positive and 15 Gram-negative anaerobes. Minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) were determined for all strains by microtitre using serial dilutions in Mueller-Hinton broth for aerobes and Brucella broth for anaerobes. The final inoculum in each well was 10(5) cfu/ml. This study shows that none of the Gram-negative organisms was sensitive to TRO; the most sensitive were streptococci aerobic strains; TRO exhibited antibiotic activity against all anaerobes tested, which was not seen with SPEC, and also exhibited bactericidal activity. The MICs of Staphylococcus epidermidis and Bacteroides fragilis tested with TRO and SPEC were not significantly affected by the size of bacterial inoculum tested, and the nature of the growth medium did not alter the susceptibility tests.
在一项体外研究中，使用一批近期临床分离菌株，将曲古霉素（TRO）的一般抗菌特性与壮观霉素（SPEC）进行了比较：50株革兰氏阳性需氧菌、25株革兰氏阴性需氧菌、30株革兰氏阳性厌氧菌和15株革兰氏阴性厌氧菌。通过微量滴定法测定所有菌株的最低抑菌浓度（MIC）和最低杀菌浓度（MBC），需氧菌在 Mueller-Hinton 肉汤中、厌氧菌在布鲁氏菌肉汤中进行系列稀释。每个孔中的最终接种量为10(5) cfu/ml。该研究表明，革兰氏阴性菌对TRO均不敏感；最敏感的是需氧链球菌菌株；TRO对所有测试的厌氧菌均表现出抗生素活性，而SPEC则未观察到这种活性，并且TRO还表现出杀菌活性。用TRO和SPEC测试的表皮葡萄球菌和脆弱拟杆菌的MIC不受测试细菌接种量大小的显著影响，生长培养基的性质也未改变药敏试验结果。
