[茅苍术中苍术酮、苍术素、β-桉叶醇、苍术素醇的同时测定及系统聚类分析]

[Simultaneous determination of atractylone, hinesol, beta-eudesmol, atrctylodin in Atractylodes lancea and hierarchical cluster analysis].

作者信息

Zhang Lei, Ouyang Zhen, Zhao Ming, Wang Peixiang, Fang Jing

机构信息

School of Pharmacy, Jiangsu University, Zhenjiang 212013, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2010 Mar;35(6):725-8. doi: 10.4268/cjcmm20100615.

DOI:10.4268/cjcmm20100615

PMID:20545197

Abstract

OBJECTIVE

To develop a GC method for simultaneous determination of 4 compounds (atractylone, hinesol, beta-eudesmol and atractylodin) in Atractylodes lancea.

METHOD

A HP-1 capillary column (0.25 mm x 30 m, 0.25 microm) was used. The detector was FID:Inlet temperature was 250 degrees C. The detector temperature was 250 degrees C. The column temperature was set at 145 degrees C and held for 25 min after injection, then programmed at 10 degrees C x min(-1) to 250 degrees C and held for 10 min at the temperature. The carrying gas was nitrogen, split ratio was 40:1. Injection volume was 2 microL, Cluster analysis was performed by SPSS13.0 software.

RESULT

The linear ranges for atractylone, hinesol, beta-eudesmol and atractylodin were 0.0122. 32 (r = .9998), 0.008-1.68 (r = 0.9998), 0.009-1.76 (r = 0.9999), 0.016-3.20 g x L(-1) (r = 0.9997), respectively. The average recoveries (n = 3) of atractylone, hinesol, beta-eudesmol and atractylodin were 98.0%-99.0%, 97.7%-99.4%, 98.4%-99.2%, 97.8%-99.7%, respectively. The samples analyzed were divided into two classes.

CONCLUSION

This method is simple, specific, repeatable and stable. It can be applied for the simultaneous determination of 4 compounds (atractylone, hinesol, beta-eudesmol and atractylodin) in A. lancea, which will provide the basis for the quality control of A. lancea. The contents of 4 active compounds were significantly different between geo-authentic and non-authentic producing areas.

摘要

目的

建立同时测定茅苍术中4种成分（苍术酮、苍术素、β-桉叶醇和苍术素醇）的气相色谱法。

方法

采用HP-1毛细管柱（0.25mm×30m，0.25μm）。检测器为FID；进样口温度为250℃，检测器温度为250℃。柱温设定为145℃，进样后保持25min，然后以10℃/min的速率程序升温至250℃，并在该温度下保持10min。载气为氮气，分流比为40:1。进样量为2μL，用SPSS13.0软件进行聚类分析。

结果

苍术酮、苍术素、β-桉叶醇和苍术素醇的线性范围分别为0.012～2.32（r = 0.9998）、0.008～1.68（r = 0.9998）、0.009～1.76（r = 0.9999）、0.016～3.20g/L（r = 0.9997）。苍术酮、苍术素、β-桉叶醇和苍术素醇的平均回收率（n = 3）分别为98.0%～99.0%、97.7%～99.4%、98.4%～99.2%、97.8%～99.7%。所分析的样品分为两类。