Rossman T G, Molina M, Meyer L, Boone P, Klein C B, Wang Z, Li F, Lin W C, Kinney P L
Institute of Environmental Medicine, NYU Medical Center, Tuxedo 10987.
Mutat Res. 1991 Aug;260(4):349-67. doi: 10.1016/0165-1218(91)90021-d.
The Microscreen assay was developed as a means of testing very small samples, as in complex mixture fractionation. It is a multi-endpoint assay which utilizes E. coli WP2s(lambda). Exposure takes place to serial dilutions of the test compound in microtitre wells (250 microliters) followed by sampling from wells in which growth has occurred ('non-toxic wells'). Although a number of different endpoints can be measured, only the prophage induction endpoint (the first one developed) has been extensively tested. Results with 133 compounds are presented. These include 111 compounds which have been tested in the S. typhimurium assay and 66 compounds for which both rodent bioassay and S. typhimurium assay data exists. The concordance for the Microscreen assay and the S. typhimurium assay was 71%. For this group of compounds, the sensitivity of the Microscreen assay in detecting carcinogens was 76% compared with 58% for the S. typhimurium assay. However, the S. typhimurium assay was somewhat more specific (69%) compared with the Microscreen (56%). The overall association between carcinogenicity and Microscreen results was statistically significant (p = 0.029), whereas for the S. typhimurium assay the association with carcinogenicity was non-significant (p = 0.086). The Microscreen assay was able to detect halogenated compounds better than the S. typhimurium assay. The Microscreen assay should prove useful in complex mixture fractionation, or in other situations where sample size is limiting.
微筛检测法是作为一种检测极小样本的方法而开发的，如在复杂混合物分级分离中。它是一种多终点检测法，利用大肠杆菌WP2s(λ)。检测化合物在微量滴定孔（250微升）中进行系列稀释，然后从有生长现象的孔（“无毒孔”）中取样。虽然可以测量许多不同的终点，但只有噬菌体诱导终点（最早开发的那个）经过了广泛测试。给出了133种化合物的结果。其中包括111种已在鼠伤寒沙门氏菌检测中测试过的化合物，以及66种既有啮齿动物生物检测数据又有鼠伤寒沙门氏菌检测数据的化合物。微筛检测法与鼠伤寒沙门氏菌检测法的一致性为71%。对于这组化合物，微筛检测法检测致癌物的灵敏度为76%，而鼠伤寒沙门氏菌检测法为58%。然而，鼠伤寒沙门氏菌检测法比微筛检测法（56%）更具特异性（69%）。致癌性与微筛检测结果之间的总体关联具有统计学意义（p = 0.029），而对于鼠伤寒沙门氏菌检测法，与致癌性的关联不显著（p = 0.086）。微筛检测法检测卤代化合物的能力优于鼠伤寒沙门氏菌检测法。微筛检测法在复杂混合物分级分离或其他样本量受限的情况下应该会很有用。
