利用果蝇中的诱导性RNA干扰进行功能分析。
Functional analysis by inducible RNA interference in Drosophila melanogaster.
作者信息
Matsushima Yuichi, Adán Cristina, Garesse Rafael, Kaguni Laurie S
机构信息
Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, USA.
出版信息
Methods Mol Biol. 2007;372:207-17. doi: 10.1007/978-1-59745-365-3_15.
Abstract
Ribonucleic acid (RNA) interference triggered by double-stranded RNA has become a powerful tool for generating loss-of-function phenotypes. It is used to inactivate genes of interest and represents an elegant approach to genome functional analysis by reverse genetics. In Drosophila, RNA interference has been used in both cell culture and animals. We have adopted this approach to reveal the physiological roles of a number of proteins involved in mitochondrial deoxyribonucleic acid metabolism, and present here experimental schemes to induce the stable expression of double-stranded RNA in Schneider cells and in transgenic Drosophila.
摘要
由双链RNA引发的核糖核酸（RNA）干扰已成为产生功能缺失表型的强大工具。它被用于使感兴趣的基因失活，是一种通过反向遗传学进行基因组功能分析的巧妙方法。在果蝇中，RNA干扰已应用于细胞培养和动物实验。我们采用这种方法揭示了许多参与线粒体脱氧核糖核酸代谢的蛋白质的生理作用，并在此展示了在施耐德细胞和转基因果蝇中诱导双链RNA稳定表达的实验方案。
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