Persson G Rutger, Hitti Jane, Paul Katie, Hirschi Regula, Weibel Marianne, Rothen Marilynn, Persson Rigmor E
Department of Periodontology and Fixed Prosthodontics, Division of Oral Microbiology, School of Dental Medicine, University of Bern, Freiburgstrasse 7, Bern, Switzerland.
J Periodontol. 2008 Mar;79(3):508-16. doi: 10.1902/jop.2008.070350.
Information on the subgingival microbiota in parous women is limited. The present study assessed 74 bacterial species at periodontal sites.
Subgingival bacterial plaque was collected from women > or =6 months after delivery. Bacteria were assessed by the checkerboard DNA-DNA hybridization method. Gingivitis was defined as > or =20% of sites with bleeding on probing (BOP), and periodontitis was defined as radiographic evidence of bone loss and probing depths > or =5.0 mm.
A total of 197 women (mean age: 29.4 +/- 6.8 years; range: 18 to 46 years) were included in the study. Gingivitis was identified in 82 of 138 subjects without evidence of periodontitis (59.4%). Periodontitis was found in 59 women (32%). Higher bacterial levels in subjects with gingivitis compared to those without evidence of gingivitis were observed for Actinomyces neuii, Bifidobacterium bifidum, Corynebacterium pseudogenitalis, Porphyromonas endodontalis, Prevotella bivia, and Pseudomonas aeruginosa (P <0.001 for each). Higher bacterial levels in subjects with periodontitis compared to those without periodontitis (BOP not accounted for) were found for 32 of 79 species (P <0.001) including Lactobacillus iners, Haemophilus influenzae, Porphyromonas gingivalis, Tannerella forsythia (previously T. forsythensis), Prevotella bivia, P. aeruginosa, and Staphylococcus aureus. Binary univariate logistic regression analysis identified that P. aeruginosa (P <0.001) and T. forsythia (P <0.05) were independently predictive of periodontal status. The odds ratio of having P. aeruginosa at levels > or =1 x 10(5) in the sample and periodontitis was 3.1 (95% confidence interval: 1.6 to 5.9; P <0.001).
In addition to P. gingivalis and T. forsythia, a diverse microbiota, including P. aeruginosa, P. endodontalis, P. bivia, and S. aureus, can be found in subgingival plaque samples from women of child-bearing age with periodontitis.
关于经产妇龈下微生物群的信息有限。本研究评估了牙周部位的74种细菌。
在分娩后≥6个月的女性中收集龈下菌斑。采用棋盘式DNA-DNA杂交法评估细菌。牙龈炎定义为探诊出血（BOP）部位≥20%，牙周炎定义为有骨质流失的影像学证据且探诊深度≥5.0mm。
共有197名女性（平均年龄：29.4±6.8岁；范围：18至46岁）纳入本研究。138名无牙周炎证据的受试者中有82名患有牙龈炎（59.4%）。59名女性患有牙周炎（32%）。与无牙龈炎证据的受试者相比，在患牙龈炎的受试者中观察到纽氏放线菌、双歧双歧杆菌、假生殖器棒状杆菌、牙髓卟啉单胞菌、二路普雷沃菌和铜绿假单胞菌的细菌水平更高（每种细菌P<0.001）。与无牙周炎（不考虑BOP）的受试者相比，在79种细菌中的32种细菌中，患牙周炎的受试者的细菌水平更高（P<0.001），包括惰性乳杆菌、流感嗜血杆菌、牙龈卟啉单胞菌、福赛坦纳菌（以前称为福赛斯坦纳菌）、二路普雷沃菌、铜绿假单胞菌和金黄色葡萄球菌。二元单变量逻辑回归分析确定，铜绿假单胞菌（P<0.001）和福赛坦纳菌（P<0.05）可独立预测牙周状况。样本中铜绿假单胞菌水平≥1×10⁵且患有牙周炎的比值比为3.1（95%置信区间：1.6至5.9；P<0.001）。
除牙龈卟啉单胞菌和福赛坦纳菌外，在患有牙周炎的育龄女性的龈下菌斑样本中还可发现多种微生物群，包括铜绿假单胞菌、牙髓卟啉单胞菌、二路普雷沃菌和金黄色葡萄球菌。
