Berner I, Greiner M, Metzger J, Jung G, Winkelmann G
Institut für Biologie, Mikrobiologie/Biotechnologie, Universität Tübingen, Federal Republic of Germany.
Biol Met. 1991;4(2):113-8. doi: 10.1007/BF01135388.
The production of catecholate siderophores was studied in some selected species of Enterobacter (Enterobacteriaceae). The extracted catecholates were separated as iron-free compounds by HPLC on a C18 reversed-phase column using methanol/0.1% phosphoric acid or methanol/0.1% formic acid as a solvent system and identified by ion spray mass spectrometry (LC/MS, MS/MS). Five catecholate compounds were identified which include 2,3-dihydroxybenzoylserine, its linear dimer and trimer, the cyclic enterobactin and an unidentified isomer of enterobactin. In addition, a new large-scale method for the isolation of catecholate siderphores is described which is based on adsorption on XAD-2 and subsequent purification on Sephadex LH20.
在一些选定的肠杆菌属（肠杆菌科）物种中研究了儿茶酚型铁载体的产生。通过高效液相色谱法（HPLC）在C18反相柱上，使用甲醇/0.1%磷酸或甲醇/0.1%甲酸作为溶剂系统，将提取的儿茶酚类物质分离为无铁化合物，并通过离子喷雾质谱法（LC/MS、MS/MS）进行鉴定。鉴定出了五种儿茶酚类化合物，包括2,3-二羟基苯甲酰丝氨酸、其线性二聚体和三聚体、环状肠杆菌素以及一种未鉴定的肠杆菌素异构体。此外，还描述了一种新的大规模分离儿茶酚型铁载体的方法，该方法基于在XAD-2上的吸附以及随后在Sephadex LH20上的纯化。
