Wang R
Institute of Basic Medical Sciences, Beijing.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 1991 Jun;13(3):181-4.
Human adrenal gland and aldosterone-producing adenoma (APA) obtained from adrenalectomy could be maintained in vitro for one to two months. The explants continued to secrete aldosterone for the duration of culture. This method was superior to monolayer cell culture. The influence of alpha-human atrial natriuretic factor (alpha-hANF) was investigated by treating cultures of normal adrenal cortex and APA with alpha-hANF (10(-8) mol/L and 3 x 10(-8) mol/L respectively). We measured aldosterone in culture media collected at 2h before alpha-hANF treatment and 0.5, 2, 4 and 24h after. It was found that alpha-hANF stimulated aldosterone secretion of normal adrenal tissue but slightly inhibited that of APA.
从肾上腺切除术中获取的人肾上腺及醛固酮分泌性腺瘤（APA）可在体外维持培养一到两个月。外植体在培养期间持续分泌醛固酮。该方法优于单层细胞培养。通过分别用α-人心房钠尿肽（α-hANF，10⁻⁸ mol/L和3×10⁻⁸ mol/L）处理正常肾上腺皮质和APA的培养物，研究了α-人心房钠尿肽（α-hANF）的影响。我们测量了在α-hANF处理前2小时以及处理后0.5、2、4和24小时收集的培养基中的醛固酮。结果发现，α-hANF刺激正常肾上腺组织的醛固酮分泌，但对APA的醛固酮分泌略有抑制。
