Higuchi K, Nawata H, Kato K, Ibayashi H, Matsuo H
J Clin Endocrinol Metab. 1986 Jul;63(1):192-6. doi: 10.1210/jcem-63-1-192.
The effects of synthetic alpha-human atrial natriuretic polypeptide (alpha hANP) on aldosteronogenesis in normal and aldosterone-producing adenoma cells (APA cells) in primary monolayer cultures were studied. alpha hANP significantly inhibited aldosterone secretion from normal adrenal cells in culture, but had no inhibitory effect on aldosterone secretion from APA cells in the presence or absence of 10(-8) M ACTH. alpha hANP enhanced the accumulation of intracellular cGMP in normal adrenal cells in culture, but not in APA cells. Visualization of [125I]iodo-alpha hANP-specific binding sites in APA and adjacent normal adrenal tissues by an in vitro receptor autoradiographic technique showed that these sites were localized only in normal adrenal tissue, but not in APA tissue. These results suggest that the lack of an inhibitory effect of alpha hANP on aldosteronogenesis in APA cells may be due to the absence of alpha hANP-specific receptor sites in APA cells.
研究了合成的α-人心房利钠多肽（αhANP）对原代单层培养的正常及醛固酮分泌性腺瘤细胞（APA细胞）醛固酮生成的影响。αhANP显著抑制培养的正常肾上腺细胞分泌醛固酮，但在存在或不存在10^(-8) M促肾上腺皮质激素（ACTH）的情况下，对APA细胞的醛固酮分泌均无抑制作用。αhANP增强了培养的正常肾上腺细胞内cGMP的积累，但对APA细胞无此作用。通过体外受体放射自显影技术对APA及相邻正常肾上腺组织中[125I]碘-αhANP特异性结合位点进行可视化显示，这些位点仅定位于正常肾上腺组织，而不在APA组织中。这些结果表明，αhANP对APA细胞醛固酮生成缺乏抑制作用可能是由于APA细胞中缺乏αhANP特异性受体位点。
