Higuchi K, Nawata H, Kato K, Ibayashi H
Third Department of Internal Medicine, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
Horm Metab Res. 1988 Jun;20(6):360-3. doi: 10.1055/s-2007-1010836.
The effects of synthetic alpha-human atrial natriuretic polypeptide (alpha-hANP) on cortisol secretion by adrenocortical adenoma cells from patients with Cushing's syndrome (CS cells) in primary monolayer cultures, compared to cultured normal adrenal cells, were studied. alpha-hANP significantly inhibited cortisol secretion by human normal adrenal cells in culture, but had no direct effect on cortisol secretion from CS cells, in the presence or absence of 10(-8) M ACTH. alpha-hANP enhanced the accumulation of intracellular cyclic GMP in normal adrenal cells in culture, but not in CS cells. Visualization of [125I] iodo-alpha-hANP-specific binding sites by an in vitro receptor autoradiographic technique showed that these sites were lacking in adrenocortical adenoma tissues. These results suggest that the loss of alpha-hANP inhibitory effect on cortisol secretion in CS cells may be due to the absence of alpha-hANP receptor sites.
研究了合成的α-人心房利钠多肽（α-hANP）对库欣综合征患者肾上腺皮质腺瘤细胞（CS细胞）在原代单层培养中皮质醇分泌的影响，并与培养的正常肾上腺细胞进行了比较。α-hANP显著抑制培养的人正常肾上腺细胞的皮质醇分泌，但在存在或不存在10^(-8) M促肾上腺皮质激素（ACTH）的情况下，对CS细胞的皮质醇分泌没有直接影响。α-hANP增强了培养的正常肾上腺细胞中细胞内环磷酸鸟苷（cGMP）的积累，但在CS细胞中没有。通过体外受体放射自显影技术对[125I]碘-α-hANP特异性结合位点的可视化显示，肾上腺皮质腺瘤组织中缺乏这些位点。这些结果表明，CS细胞中α-hANP对皮质醇分泌的抑制作用丧失可能是由于缺乏α-hANP受体位点。
