合成人心房利钠肽对体外培养的散在醛固酮瘤细胞醛固酮分泌的影响。

Effect of synthetic human atrial natriuretic peptide on aldosterone secretion by dispersed aldosterone-producing adenoma cells in vitro.

作者信息

Hirata Y, Tomita M, Yoshimi H, Kuramochi M, Ito K, Ikeda M

出版信息

J Clin Endocrinol Metab. 1985 Oct;61(4):677-80. doi: 10.1210/jcem-61-4-677.

Abstract

The effect of synthetic alpha-human atrial natriuretic peptide (alpha hANP), a potent natriuretic and vasorelaxant polypeptide recently isolated from human atria, on aldosterone secretion was studied in vitro in collagenase-dispersed adrenal adenoma cells from a patient with primary aldosteronism. alpha hANP (3.2 X 10(-7) M) significantly inhibited both basal and potassium (16 mM)-stimulated aldosterone secretion, whereas it had little or no effect on aldosterone secretion submaximally or maximally stimulated by ACTH (3.4 X 10(-10)-3.4 X 10(-9) M) or angiotensin II (10(-8)-10(-9) M). The less potent effect of alpha hANP on aldosterone secretion by dispersed human adrenal tumor cells compared to that in in vitro animal studies may reflect decreased affinity and/or number of specific receptors for ANP on the tumor cells. Whether ANP plays a physiological role in regulation of aldosterone secretion in humans in vivo remains to be determined.

摘要

合成的α-人心房利钠肽（α-hANP）是一种最近从人心房中分离出的具有强大利钠和血管舒张作用的多肽，我们在体外对一名原发性醛固酮增多症患者的胶原酶分散肾上腺腺瘤细胞研究了其对醛固酮分泌的影响。α-hANP（3.2×10⁻⁷M）显著抑制基础状态和钾（16mM）刺激的醛固酮分泌，而对促肾上腺皮质激素（3.4×10⁻¹⁰ - 3.4×10⁻⁹M）或血管紧张素II（10⁻⁸ - 10⁻⁹M）亚最大或最大刺激的醛固酮分泌几乎没有影响。与体外动物研究相比，α-hANP对分散的人肾上腺肿瘤细胞醛固酮分泌的作用较弱，这可能反映了肿瘤细胞上ANP特异性受体的亲和力和/或数量降低。ANP在体内是否对人类醛固酮分泌调节发挥生理作用仍有待确定。