Asker Dalal, Beppu Teruhiko, Ueda Kenji
Department of Food Science, University of Massachusetts, 100 Holdsworth Way, Amherst, MA 01003, USA.
Int J Syst Evol Microbiol. 2008 Mar;58(Pt 3):601-6. doi: 10.1099/ijs.0.65493-0.
A yellow-pigmented, Gram-negative, strictly aerobic, rod-shaped bacterium (TDMA-5(T)) was isolated from a freshwater sample collected at Misasa (Tottori, Japan). The DNA G+C content was 38.6 mol%. Major fatty acids were iso-C(15 : 0), iso-C(17 : 0) 3-OH and summed feature 4 (iso-C(15 : 0) 2-OH and/or C(16 : 1)omega7c). MK-7 was the predominant respiratory quinone. Zeaxanthin was the major carotenoid pigment produced; flexirubin-type pigments were not produced. TDMA-5(T) was sensitive to gamma-irradiation. The strain degraded gelatin, casein, starch, Tween 80 and DNA. Phylogenetic analysis based on the 16S rRNA gene sequence placed TDMA-5(T) in a distinct lineage in the family Sphingobacteriaceae, sharing 89.4-93.4 % sequence similarity with members of the nearest genus Pedobacter. Strain TDMA-5(T) could be distinguished from the other members of the family Sphingobacteriaceae by a number of chemotaxonomic and phenotypic characteristics. Based on its unique phenotypic, genotypic and phylogenetic features, strain TDMA-5(T) represents a novel genus and species, for which the name Nubsella zeaxanthinifaciens gen. nov., sp. nov. is proposed. The type strain of Nubsella zeaxanthinifaciens is TDMA-5(T) (=NBRC 102579(T) =CCUG 54348(T)).
从日本鸟取县三朝采集的淡水样本中分离出一株黄色色素沉着、革兰氏阴性、严格需氧的杆状细菌（TDMA-5(T)）。DNA G+C含量为38.6 mol%。主要脂肪酸为异-C(15 : 0)、异-C(17 : 0) 3-OH和总和特征4（异-C(15 : 0) 2-OH和/或C(16 : 1)ω7c）。MK-7是主要的呼吸醌。玉米黄质是产生的主要类胡萝卜素色素；未产生柔韧菌素类色素。TDMA-5(T)对γ射线敏感。该菌株能降解明胶、酪蛋白、淀粉、吐温80和DNA。基于16S rRNA基因序列的系统发育分析将TDMA-5(T)置于鞘氨醇杆菌科的一个独特谱系中，与最近的Pedobacter属成员的序列相似性为89.4-93.4%。菌株TDMA-5(T)可通过一些化学分类和表型特征与鞘氨醇杆菌科的其他成员区分开来。基于其独特的表型、基因型和系统发育特征，菌株TDMA-5(T)代表一个新的属和种，为此提出新属名Nubsella zeaxanthinifaciens gen. nov.，新种名Nubsella zeaxanthinifaciens sp. nov.。Nubsella zeaxanthinifaciens的模式菌株为TDMA-5(T)（=NBRC 102579(T) =CCUG 54348(T)）。
