Department of Microbiology, Key Lab of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.
College of Life Sciences, Zaozhuang University, Zaozhuang, 277160, People's Republic of China.
Curr Microbiol. 2019 Apr;76(4):442-447. doi: 10.1007/s00284-019-01643-6. Epub 2019 Feb 13.
A Gram-stain negative, strictly aerobic, non-spore forming, non-motile, rod-shaped bacterium, designated TBBPA-24, was isolated from tetrabromobisphenol A-contaminated soil in China. Phylogenetic analysis based on the 16S rRNA gene sequences showed that strain TBBPA-24 was most closely related to Pedobacter nanyangensis Q4 (96.5%) and Pedobacter 'zeaxanthinifaciens' TDMA-5 (96.1%). Chemotaxonomic analysis revealed that strain TBBPA-24 possessed MK-7 as the major respiratory quinone and lipid, aminolipid, phospholipid, phosphatidylethanolamine, and phosphoaminolipid as the major polar lipid. The major fatty acids were iso-C (40.2%), summed feature 3 (Cω6c and/or Cω7c, 25.6%) and iso-C 3-OH (16.4%). The genomic DNA G+C content of strain TBBPA-24 was 43.9 mol%. Based on the phylogenetic, phenotypic characteristics, and chemotaxonomic data, strain TBBPA-24 is considered a novel species of the genus Pedobacter, for which the name Pedobacter pollutisoli sp. nov. is proposed. The type strain TBBPA-24 (= KCTC 62314 = CCTCC AB 2017244) is proposed.
一株革兰氏阴性、严格需氧、不产芽孢、不运动、杆状的细菌，被命名为 TBBPA-24，从中国受四溴双酚 A 污染的土壤中分离得到。基于 16S rRNA 基因序列的系统发育分析表明，菌株 TBBPA-24 与 Pedobacter nanyangensis Q4（96.5%）和 Pedobacter 'zeaxanthinifaciens' TDMA-5（96.1%）最为密切相关。化学生态学分分析表明，菌株 TBBPA-24 含有 MK-7 作为主要呼吸醌和脂类，胺脂、磷脂、磷脂酰乙醇胺和磷酯酰氨基醇作为主要极性脂类。主要脂肪酸为 iso-C（40.2%）、总和特征 3（Cω6c 和/或 Cω7c，25.6%）和 iso-C 3-OH（16.4%）。菌株 TBBPA-24 的基因组 DNA G+C 含量为 43.9 mol%。基于系统发育、表型特征和化学分类学数据，菌株 TBBPA-24 被认为是 Pedobacter 属的一个新种，命名为 Pedobacter pollutisoli sp. nov.。其模式菌株为 TBBPA-24（=KCTC 62314=CCTCC AB 2017244）。
