Wang Li-Ting, Lee Fwu-Ling, Tai Chun-Ju, Kuo Hsiao-Ping
Bioresource Collection and Research Center, Food Industry Research and Development Institute, PO Box 246, Hsinchu 30099, Taiwan, ROC.
Int J Syst Evol Microbiol. 2008 Mar;58(Pt 3):671-5. doi: 10.1099/ijs.0.65191-0.
Strain BCRC 14193, isolated from soil, shared more than 99 % 16S rRNA gene sequence similarity with Bacillus amyloliquefaciens BCRC 11601(T) and Bacillus velezensis BCRC 17467(T). This strain was previously identified as B. amyloliquefaciens, based on DNA-DNA hybridization, but its DNA relatedness value with B. velezensis BCRC 17467(T) was 89 %. To investigate the relatedness of strain BCRC 14193, B. amyloliquefaciens and B. velezensis, the partial sequence of the gene encoding the subunit B protein of DNA gyrase (gyrB) was determined. B. velezensis BCRC 17467(T) shared high gyrB gene sequence similarity with B. amyloliquefaciens BCRC 14193 (98.4 %) and all of the B. amyloliquefaciens strains available (95.5-95.6 %). DNA-DNA hybridization experiments revealed high relatedness values between B. velezensis BCRC 17467(T) and B. amyloliquefaciens BCRC 11601(T) (74 %) and the B. amyloliquefaciens reference strains (74-89 %). Based on these data and the lack of phenotypic distinctive characteristics, we propose Bacillus velezensis as a later heterotypic synonym of Bacillus amyloliquefaciens.
从土壤中分离出的菌株BCRC 14193与解淀粉芽孢杆菌BCRC 11601(T)和贝莱斯芽孢杆菌BCRC 17467(T)的16S rRNA基因序列相似性超过99%。基于DNA-DNA杂交，该菌株先前被鉴定为解淀粉芽孢杆菌，但其与贝莱斯芽孢杆菌BCRC 17467(T)的DNA相关性值为89%。为了研究菌株BCRC 14193、解淀粉芽孢杆菌和贝莱斯芽孢杆菌之间的相关性，测定了编码DNA促旋酶亚基B蛋白（gyrB）的基因部分序列。贝莱斯芽孢杆菌BCRC 17467(T)与解淀粉芽孢杆菌BCRC 14193（98.4%）以及所有可用的解淀粉芽孢杆菌菌株（95.5 - 95.6%）具有较高的gyrB基因序列相似性。DNA-DNA杂交实验表明，贝莱斯芽孢杆菌BCRC 17467(T)与解淀粉芽孢杆菌BCRC 11601(T)（74%）以及解淀粉芽孢杆菌参考菌株（74 - 89%）之间具有较高的相关性值。基于这些数据以及缺乏表型独特特征，我们提议将贝莱斯芽孢杆菌作为解淀粉芽孢杆菌的后选异名。
