Folmsbee Martha, Duncan Kathleen, Han Sung Ok, Nagle David, Jennings Eleanor, McInerney Michael
University of Oklahoma, Norman, OK 73019, USA.
Syst Appl Microbiol. 2006 Dec;29(8):645-9. doi: 10.1016/j.syapm.2006.01.010. Epub 2006 Feb 20.
Bacillus strain JF-2 (ATCC 39307) is a halotolerant, biosurfactant-producing bacterium that was initially described as a member of the species Bacillus licheniformis based on a limited set of phenotypic characteristics. Here, genetic and phenotypic analyses were employed to determine the relationship of Bacillus strain JF-2 to other Bacillus strains. The restriction patterns with AluI and analysis of gyrA and 16S rRNA gene sequences grouped Bacillus strain JF-2 with B. mojavensis(T) and not with B. licheniformis(T). DNA-DNA similarity showed JF-2 was 75% similar to B. mojavensis(T) and only 11% similar to B. licheniformis(T). Both strain JF-2 and B. mojavensis(T) required DNA for anaerobic growth, but B. licheniformis(T) did not. B. mojavensis(T) and strain JF-2 did not grow anaerobically in thioglycollate medium or aerobically with propionate while B. licheniformis(T) grew under these conditions. DNA-DNA similarity, gene sequence data and phenotypic characteristics all support the assignment of JF-2 as a member of the species B. mojavensis.
芽孢杆菌菌株JF-2（ATCC 39307）是一种耐盐、能产生生物表面活性剂的细菌，最初基于有限的一组表型特征被描述为地衣芽孢杆菌物种的一个成员。在此，采用遗传和表型分析来确定芽孢杆菌菌株JF-2与其他芽孢杆菌菌株的关系。用AluI进行的限制性酶切图谱分析以及gyrA和16S rRNA基因序列分析将芽孢杆菌菌株JF-2与莫哈韦芽孢杆菌（模式菌株）归为一组，而不是与地衣芽孢杆菌（模式菌株）归为一组。DNA-DNA相似性表明JF-2与莫哈韦芽孢杆菌（模式菌株）的相似性为75%，与地衣芽孢杆菌（模式菌株）的相似性仅为11%。菌株JF-2和莫哈韦芽孢杆菌（模式菌株）厌氧生长都需要DNA，但地衣芽孢杆菌（模式菌株）则不需要。莫哈韦芽孢杆菌（模式菌株）和菌株JF-2在硫代乙醇酸盐培养基中不能厌氧生长，在以丙酸盐为底物时也不能好氧生长，而地衣芽孢杆菌（模式菌株）在这些条件下能够生长。DNA-DNA相似性、基因序列数据和表型特征均支持将JF-2归为莫哈韦芽孢杆菌物种的一个成员。
