Carvalho Maria F, De Marco Paolo, Duque Anouk F, Pacheco Catarina C, Janssen Dick B, Castro Paula M L
Escola Superior de Biotecnologia, Universidade Católica Portuguesa, Rua Dr. António Bernardino de Almeida, 4200-072 Porto, Portugal.
Int J Syst Evol Microbiol. 2008 Mar;58(Pt 3):692-8. doi: 10.1099/ijs.0.65472-0.
A detailed classification of a novel bacterial strain, designated F11(T), capable of degrading fluorobenzene as a sole carbon and energy source, was performed by using a polyphasic approach. This Gram-negative, rod-shaped, non-motile, non-spore-forming, aerobic bacterium was isolated from a sediment sample collected from an industrially contaminated site in northern Portugal. The predominant whole-cell fatty acids were C(19 : 0) cyclo omega8c, C(16 : 0), C(18 : 1)omega7c, C(18 : 0), C(18 : 0) 3-OH and C(16 : 0) 3-OH. The G+C content of the DNA was 62.9 mol% and the major respiratory quinone was ubiquinone 10 (UQ-10). 16S rRNA gene sequence analysis revealed that strain F11(T) was a member of the class Alphaproteobacteria and was phylogenetically related to the genus Labrys, having sequence similarities of 95.6 and 93.1 % to the type strains of Labrys monachus and Labrys methylaminiphilus, respectively. DNA-DNA hybridization experiments revealed levels of relatedness of <70 % between strain F11(T) and the type strains of L. monachus and L. methylaminiphilus (38.6 and 34.1 %, respectively), justifying the classification of strain F11(T) as representing a novel species of the genus Labrys. The name Labrys portucalensis sp. nov. is proposed for this organism. The type strain is F11(T) (=LMG 23412(T)=DSM 17916(T)).
采用多相分类法对一株新的细菌菌株F11(T)进行了详细分类，该菌株能够以氟苯作为唯一碳源和能源进行降解。这株革兰氏阴性、杆状、不运动、不产芽孢的需氧细菌是从葡萄牙北部一个受工业污染场地采集的沉积物样本中分离得到的。主要的全细胞脂肪酸为C(19 : 0)环ω8c、C(16 : 0)、C(18 : 1)ω7c、C(18 : 0)、C(18 : 0) 3-OH和C(16 : 0) 3-OH。DNA的G+C含量为62.9 mol%，主要呼吸醌为泛醌10（UQ-10）。16S rRNA基因序列分析表明，菌株F11(T)属于α-变形菌纲，在系统发育上与Labrys属相关，与Labrys monachus和Labrys methylaminiphilus的模式菌株的序列相似性分别为95.6%和93.1%。DNA-DNA杂交实验表明，菌株F11(T)与L. monachus和L. methylaminiphilus的模式菌株之间的相关性水平<70%（分别为38.6%和34.1%），这证明将菌株F11(T)分类为Labrys属的一个新物种是合理的。为此种生物提议命名为Labrys portucalensis sp. nov.。模式菌株为F11(T)（=LMG 23412(T)=DSM 17916(T)）。
