Description of sp. nov., isolated from a diclofenac-degrading enrichment culture, and genome-based reclassification of as a later heterotypic synonym of .

A Gram-stain-negative strain, designated as Zidic-5, was isolated from diclofenac-degrading enrichment culture and characterized using a polyphasic approach to determine its taxonomic position. The 16S rRNA gene sequence analysis revealed that strain Zidic-5 belongs to the genus , with the highest 16S rRNA gene similarity to LMG 23578 (99.13%), followed by F11 (99.06%), JLW10 (98.58%) and DSM 18385 (98.32%). The draft genome sequence of strain Zidic-5 is 7.64 Mb long, and the G+C content of the genome is 63.5 mol%. The orthologous average nucleotide identity and digital DNA-DNA hybridization relatedness values between strain Zidic-5 and its closest relatives were below the threshold values for species demarcation, confirming that strain Zidic-5 is distinctly separated from its closest relatives. Additionally, comparative whole-genome analysis of type strains of and indicated that they belong to the same genomic species, suggesting that is a later heterotypic synonym of . Cells of strain Zidic-5 were strictly aerobic, coccoid-shaped and non-motile. The predominant fatty acids (>10% of the total) of strain Zidic-5 were C ω7c, C and C cyclo ω7c. The major ubiquinone of strain Zidic-5 was Q-10, while the major polar lipids were phosphatidylcholine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and aminophospholipid. Based on the polyphasic study, it is concluded that strain Zidic-5 represents a novel species of the genus ; thus, the name of sp. nov. is proposed. The type strain of the species is strain Zidic-5 (=LMG 33565=NCAIM B.02686).