Csépányi Andrea, Táncsics András, Pápai Márton, Baka Erzsébet, Tóth Erika, Bóka Károly, Daood Hussein, Szabó István, Kriszt Balázs
Department of Molecular Ecology, Institute of Aquaculture and Environmental Safety, Hungarian University of Agriculture and Life Sciences, Gödöllő, Hungary.
Department of Microbiology, Eötvös Loránd University, Budapest, Hungary.
Int J Syst Evol Microbiol. 2025 May;75(5). doi: 10.1099/ijsem.0.006778.
A Gram-stain-negative strain, designated as Zidic-5, was isolated from diclofenac-degrading enrichment culture and characterized using a polyphasic approach to determine its taxonomic position. The 16S rRNA gene sequence analysis revealed that strain Zidic-5 belongs to the genus , with the highest 16S rRNA gene similarity to LMG 23578 (99.13%), followed by F11 (99.06%), JLW10 (98.58%) and DSM 18385 (98.32%). The draft genome sequence of strain Zidic-5 is 7.64 Mb long, and the G+C content of the genome is 63.5 mol%. The orthologous average nucleotide identity and digital DNA-DNA hybridization relatedness values between strain Zidic-5 and its closest relatives were below the threshold values for species demarcation, confirming that strain Zidic-5 is distinctly separated from its closest relatives. Additionally, comparative whole-genome analysis of type strains of and indicated that they belong to the same genomic species, suggesting that is a later heterotypic synonym of . Cells of strain Zidic-5 were strictly aerobic, coccoid-shaped and non-motile. The predominant fatty acids (>10% of the total) of strain Zidic-5 were C ω7c, C and C cyclo ω7c. The major ubiquinone of strain Zidic-5 was Q-10, while the major polar lipids were phosphatidylcholine, diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and aminophospholipid. Based on the polyphasic study, it is concluded that strain Zidic-5 represents a novel species of the genus ; thus, the name of sp. nov. is proposed. The type strain of the species is strain Zidic-5 (=LMG 33565=NCAIM B.02686).
从双氯芬酸降解富集培养物中分离出一株革兰氏阴性菌株，命名为Zidic-5，并采用多相分类法对其进行表征，以确定其分类地位。16S rRNA基因序列分析表明，菌株Zidic-5属于 属，与LMG 23578的16S rRNA基因相似度最高（99.13%），其次是F11（99.06%）、JLW10（98.58%）和DSM 18385（98.32%）。菌株Zidic-5的基因组草图序列长7.64 Mb，基因组的G+C含量为63.5 mol%。菌株Zidic-5与其最接近的亲缘种之间的直系同源平均核苷酸同一性和数字DNA-DNA杂交相关性值低于物种划分的阈值，证实菌株Zidic-5与其最接近的亲缘种明显不同。此外，对 和 的模式菌株进行的全基因组比较分析表明，它们属于同一基因组物种，这表明 是 的晚出异名。菌株Zidic-5的细胞严格需氧，呈球状且无运动性。菌株Zidic-5的主要脂肪酸（占总量的>10%）为C ω7c、C 和C环ω7c。菌株Zidic-5的主要泛醌为Q-10，而主要极性脂为磷脂酰胆碱、二磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺和氨基磷脂。基于多相研究，得出结论：菌株Zidic-5代表 属的一个新物种；因此，提出 新种的名称。该物种的模式菌株是菌株Zidic-5（=LMG 33565=NCAIM B.02686）。
