Lai Qiliang, Yuan Jun, Gu Li, Shao Zongze
Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, PR China.
Int J Syst Evol Microbiol. 2009 Jun;59(Pt 6):1278-81. doi: 10.1099/ijs.0.003889-0.
A taxonomic study was carried out on strain B142(T), which was isolated from a crude-oil-degrading microbial consortium via enrichment with deep water from the Indian Ocean. Cells of the isolate were Gram-negative, oxidase-negative, catalase-positive, helical in shape, motile by means of polar flagella (three per cell) and moderately halophilic. Growth was observed at salinities of 0.5-12 % and at temperatures of 10-41 degrees C. The micro-organism was capable of denitrification, but was unable to degrade Tween 80 or gelatin. The predominant fatty acids were C(16 : 1)omega7c and/or iso-C(15 :0 )2-OH (6.4 %), C(16 : 0) (15.7 %), C(18 : 1)omega7c (45 %), C(18 : 0) (6.8 %) and C(19 : 0)omega8c cyclo (6.7 %). The G+C content of the chromosomal DNA was 67.3 mol%. Comparisons of 16S rRNA gene sequences showed that strain B142(T) was most closely related to the type strains of two Insolitispirillum peregrinum subspecies (93.0-93.1 % sequence similarity), two Novispirillum itersonii subspecies (92.8-92.9 %) and Caenispirillum bisanense (91.7 %); sequence similarities with respect to other taxa were below 90.5 %. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain B142(T) formed a distinct evolutionary lineage within the family Rhodospirillaceae. Strain B142(T) was distinguishable from phylogenetically related genera with regard to several phenotypic properties. On the basis of phenotypic and phylogenetic data, therefore, strain B142(T) represents a novel genus and species, for which the name Marispirillum indicum gen. nov., sp. nov. is proposed. The type strain is B142(T) (=CCTCC AB 208225(T)=LMG 24627(T)=MCCC 1A01235(T)).
对菌株B142(T)进行了分类学研究，该菌株是从一个原油降解微生物群落中分离得到的，通过用印度洋深水进行富集培养。该分离菌株的细胞为革兰氏阴性、氧化酶阴性、过氧化氢酶阳性、呈螺旋状、通过极生鞭毛运动（每个细胞三根）且为中度嗜盐菌。在盐度为0.5 - 12%和温度为10 - 41℃时观察到生长。该微生物能够进行反硝化作用，但不能降解吐温80或明胶。主要脂肪酸为C(16 : 1)ω7c和/或异-C(15 :0 )2-OH (6.4%)、C(16 : 0) (15.7%)、C(18 : 1)ω7c (45%)、C(18 : 0) (6.8%)和C(19 : 0)ω8c环 (6.7%)。染色体DNA的G+C含量为67.3 mol%。16S rRNA基因序列比较表明，菌株B142(T)与两个奇异螺旋菌亚种的模式菌株关系最为密切（序列相似性为93.0 - 93.1%）、两个迭代新螺旋菌亚种（92.8 - 92.9%）以及比桑海生螺旋菌（91.7%）；与其他分类单元的序列相似性低于90.5%。基于16S rRNA基因序列的系统发育分析表明，菌株B142(T)在红螺菌科内形成了一个独特的进化分支。菌株B142(T)在几个表型特征方面与系统发育相关属不同。因此，基于表型和系统发育数据，菌株B142(T)代表一个新的属和种，为此提出新属名印度洋海螺旋菌属（Marispirillum），新种名印度洋海螺旋菌（Marispirillum indicum）。模式菌株为B142(T)（=CCTCC AB 208225(T)=LMG 24627(T)=MCCC 1A01235(T)）。
