Xiu Zhi-Long, Zeng An-Ping
Department of Bioscience and Biotechnology, School of Environmental and Biological Science and Technology, Dalian University of Technology, Dalian, PR China.
Appl Microbiol Biotechnol. 2008 Apr;78(6):917-26. doi: 10.1007/s00253-008-1387-4. Epub 2008 Mar 5.
1,3-Propanediol and 2,3-butanediol are two promising chemicals which have a wide range of applications and can be biologically produced. The separation of these diols from fermentation broth makes more than 50% of the total costs in their microbial production. This review summarizes the present state of methods studied for the recovery and purification of biologically produced diols, with particular emphasis on 1,3-propoanediol. Previous studies on the separation of 1,3-propanediol primarily include evaporation, distillation, membrane filtration, pervaporation, ion exchange chromatography, liquid-liquid extraction, and reactive extraction. Main methods for the recovery of 2,3-butanediol include steam stripping, pervaporation, and solvent extraction. No single method has proved to be simple and efficient, and improvements are especially needed with regard to yield, purity, and energy consumption. Perspectives for an improved downstream processing of biologically produced diols, especially 1,3-propanediol are discussed based on our own experience and recent work. It is argued that separation technologies such as aqueous two-phase extraction with short chain alcohols, pervaporation, reverse osmosis, and in situ extractive or pervaporative fermentations deserve more attention in the future.
1,3 - 丙二醇和2,3 - 丁二醇是两种很有前景的化学品，具有广泛的应用，且可通过生物法生产。从发酵液中分离这些二醇的成本占其微生物生产总成本的50%以上。本文综述了生物法生产二醇的回收和纯化方法的研究现状，特别着重于1,3 - 丙二醇。以往关于1,3 - 丙二醇分离的研究主要包括蒸发、蒸馏、膜过滤、渗透蒸发、离子交换色谱、液 - 液萃取和反应萃取。回收2,3 - 丁二醇的主要方法包括汽提、渗透蒸发和溶剂萃取。尚无单一方法被证明既简单又高效，尤其在产率、纯度和能耗方面还需要改进。基于我们自己的经验和近期工作，讨论了生物法生产二醇（特别是1,3 - 丙二醇）改进下游加工的前景。有人认为，诸如用短链醇进行双水相萃取、渗透蒸发、反渗透以及原位萃取或渗透蒸发发酵等分离技术在未来值得更多关注。
