Department of Microbiology, University of Delhi South Campus, New Delhi, India.
Appl Microbiol Biotechnol. 2011 May;90(4):1267-76. doi: 10.1007/s00253-011-3161-2. Epub 2011 Mar 1.
In this paper, a downstream process for purification of 1,3-propanediol from glycerol-based fermentation broth was investigated. The purification of 1,3-propanediol from fermentation broth was achieved by a process combining microfiltration, charcoal treatment, vacuum distillation, and silica gel chromatography. The broth was first filtered through hollow fiber cartridge, wherein 98.7% of biomass was removed. Soluble proteins and other color impurities in the broth were removed by the use of activated charcoal at optimal concentration of 30 g l(-1) where the soluble proteins in the broth decreased to 0.1 g l(-1) (96.0% protein loss). The obtained broth when concentrated by vacuum distillation resulted in the crystallization of inorganic salts. Subsequently, 1,3-propanediol was purified by gradient chromatography using silica gel as a stationary phase and mixture of chloroform and methanol as a mobile phase. Finally, with the optimal flow rate of 10 ml min(-1) and loading amount of 80 ml, the yield of 1,3-propanediol achieved was 89%. The overall yield of 1,3-propanediol using the proposed procedure was 75.47%. The developed method was found to be a simple, rapid, and efficient procedure for the purification of 1,3-propanediol from fermentation broth.
本文研究了从甘油发酵液中提取 1,3-丙二醇的下游工艺。通过微滤、活性炭处理、真空蒸馏和硅胶色谱相结合的方法，从发酵液中提取 1,3-丙二醇。发酵液首先通过中空纤维筒式过滤器过滤，其中 98.7%的生物质被去除。在最佳浓度为 30 g/L 的活性炭作用下，发酵液中的可溶性蛋白和其他颜色杂质被去除，发酵液中的可溶性蛋白降低至 0.1 g/L（96.0%的蛋白损失）。获得的发酵液经真空蒸馏浓缩后，无机盐结晶。随后，1,3-丙二醇通过硅胶作为固定相和氯仿和甲醇混合物作为流动相的梯度色谱法进行纯化。最后，在最优流速为 10 ml/min 和装样量为 80 ml 的条件下，1,3-丙二醇的收率达到 89%。采用所提出的方法，1,3-丙二醇的总收率为 75.47%。研究结果表明，该方法是一种从发酵液中提取 1,3-丙二醇的简单、快速、高效的方法。
