Kumar Santosh, Agyeman-Duah Eric, Ujor Victor C
Metabolic Engineering and Fermentation Science Group, Department of Food Science, University of Wisconsin-Madison, Babcock Hall, 1605 Linden Drive, Madison, WI 53706, USA.
Bioengineering (Basel). 2023 Sep 16;10(9):1090. doi: 10.3390/bioengineering10091090.
is part of the complex (ECC), which is widespread in nature. It is a facultative Gram-negative bacterium of medical and industrial importance. We assessed the metabolic and genetic repertoires of a new isolate. Here, we report the whole-genome sequence of a furfural- and 5-hydroxymethyl furfural (HMF)-tolerant strain of (UW0SKVC1), which uses glucose, glycerol, xylose, lactose and arabinose as sole carbon sources. This strain exhibits high tolerance to furfural (IC = 34.2 mM; ~3.3 g/L) relative to DH5α (IC = 26.0 mM; ~2.5 g/L). Furfural and HMF are predominantly converted to their less-toxic alcohols. UW0SKVC1 produces 2,3-butanediol, acetoin, and acetol, among other compounds of industrial importance. UW0SKVC1 produces as high as ~42 g/L 2,3-butanediol on 60 g/L glucose or lactose. The assembled genome consists of a 4,833,490-bp chromosome, with a GC content of 55.35%. Annotation of the assembled genome revealed 4586 coding sequences and 4516 protein-coding genes (average length 937-bp) involved in central metabolism, energy generation, biodegradation of xenobiotic compounds, production of assorted organic compounds, and drug resistance. UW0SKVC1 shows considerable promise as a biocatalyst and a genetic repository of genes whose protein products may be harnessed for the efficient bioconversion of lignocellulosic biomass, abundant glycerol and lactose-replete whey permeate to value-added chemicals.
是复合体（ECC）的一部分，该复合体在自然界广泛存在。它是一种具有医学和工业重要性的兼性革兰氏阴性菌。我们评估了一株新分离株的代谢和基因组成。在此，我们报告了一株耐糠醛和5-羟甲基糠醛（HMF）的菌株（UW0SKVC1）的全基因组序列，该菌株利用葡萄糖、甘油、木糖、乳糖和阿拉伯糖作为唯一碳源。相对于DH5α（IC = 26.0 mM；约2.5 g/L），该菌株对糠醛表现出高耐受性（IC = 34.2 mM；约3.3 g/L）。糠醛和HMF主要转化为毒性较小的醇类。UW0SKVC1产生2,3-丁二醇、3-羟基丁酮和丙酮醇以及其他具有工业重要性的化合物。在60 g/L葡萄糖或乳糖上，UW0SKVC1产生高达约42 g/L的2,3-丁二醇。组装后的基因组由一条4,833,490 bp的染色体组成，GC含量为55.35%。对组装基因组的注释揭示了4586个编码序列和4516个蛋白质编码基因（平均长度937 bp），这些基因参与中心代谢、能量产生、外源化合物的生物降解、各种有机化合物的生产以及耐药性。UW0SKVC1作为一种生物催化剂和基因库具有很大的潜力，其蛋白质产物可用于将木质纤维素生物质、丰富的甘油和富含乳糖的乳清渗透物高效生物转化为增值化学品。
