Zhe Jing, Zhang Jun, Chen Shiling, Zhang Weiqing, Luo Chen, Zhou Xingyu, Chen Xin, Qiu Zhuolin, Li Huixi, Wu Xiaomin
Center for Reproductive Medicine, Department of Obstetrics and Gynecology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2019 Jul 30;39(7):766-771. doi: 10.12122/j.issn.1673-4254.2019.07.03.
To explore the causes of oocyte vitrification and its application in assisted reproduction.
We retrospectively analyzed the data of 26 patients with 27 cycles of oocyte vitrification cryopreservation undergoing intracytoplasmic sperm injection (ICSI) and embryo transfer between January, 2008 and October, 2018. The causes of oocyte vitrification and the outcomes of ICSI and clinical pregnancy were analyzed.
The causes of oocytes vitrification included mainly azoospermia or severe spermatogenesis disorder of the husband, failure to obtain sperms from the husband, failure of the husband to be present on the day of oocyte retrieval and acute diseases of the husband to not allow sperm collection. A total of 274 oocytes were frozen in 27 oocyte retrieval cycles, and 217 eggs were thawed in 19 cycles with a survival rate of 81.11% (176/217). The normal fertilization rate, cleavage rate and high-quality embryo rate was 74.81% (98/131), 89.80% (88/98) and 36.73% (36/98), respectively. Fifteen patients underwent embryo transfer, and the clinical pregnancy rate and live birth rate was 53.33% (8/15) and 33.33% (5/15), respectively. Compared with patients below 35 years of age, the patients aged above 35 years had significantly lower oocyte survival rate after thawing (82.76% 74.42%, =0.211), clinical pregnancy rate (77.78% 16.67%, =0.041) and live birth rate (55.56% 0, =0.044).
Oocytes vitrification can be used as a remedy for infertile couples who fail to provide sperms due to male factors on the day of oocyte retrieval. Vitrification of the oocytes does not significantly affect the fertilization rate or the clinical pregnancy rate. The survival rate of the thawed oocytes is related to the age of the wife, and an age younger than 35 years can be optimal for achieving favorable clinical pregnancy outcomes after oocyte vitrification.
探讨卵母细胞玻璃化冷冻的原因及其在辅助生殖中的应用。
回顾性分析2008年1月至2018年10月期间26例患者27个周期进行卵母细胞玻璃化冷冻保存并行卵胞浆内单精子注射（ICSI）及胚胎移植的数据。分析卵母细胞玻璃化冷冻的原因及ICSI结局和临床妊娠情况。
卵母细胞玻璃化冷冻的原因主要包括男方无精子症或严重精子发生障碍、未能从男方获取精子、取卵日男方未到场及男方急性疾病无法采集精子。27个取卵周期共冷冻274枚卵母细胞，19个周期解冻217枚卵，复苏率为81.11%（176/217）。正常受精率、卵裂率和优质胚胎率分别为74.81%（98/131）、89.80%（88/98）和36.73%（36/98）。15例患者进行胚胎移植，临床妊娠率和活产率分别为53.33%（8/15）和33.33%（5/15）。与35岁以下患者相比，35岁及以上患者解冻后卵母细胞存活率（82.76%对74.42%，P = 0.211）、临床妊娠率（77.78%对16.67%，P = 0.041）和活产率（55.56%对0，P = 0.044）显著降低。
卵母细胞玻璃化冷冻可作为取卵日因男方因素无法提供精子的不孕夫妇的补救措施。卵母细胞玻璃化冷冻对受精率和临床妊娠率无显著影响。解冻后卵母细胞的存活率与女方年龄有关，35岁以下可能是卵母细胞玻璃化冷冻后获得良好临床妊娠结局的最佳年龄。
