Koike-Kiriyama Naoko, Adachi Yasushi, Minamino Keizo, Iwasaki Masayoshi, Nakano Keiji, Koike Yasushi, Yamada Haruhiko, Mukaide Hiromi, Shigematsu Akio, Mizokami Tomomi, Matsumura Miyo, Ikehara Susumu
First Department of Pathology, Kansai Medical University, Moriguchi City, Osaka 570-8506, Japan.
Acta Neurobiol Exp (Wars). 2007;67(4):359-65. doi: 10.55782/ane-2007-1653.
Retinal degeneration and dystrophy are the major causes of blindness in the developed world. It has been reported that human cord blood cells (HCBCs) can differentiate into neuron-like cells in vitro. We have recently demonstrated that bone marrow cells (BMCs) of both mice and rats can differentiate into retinal nerve cells (RNCs). In the present study, we show the differentiation capacity of HCBCs into RNCs in vivo. We transplanted lineage-negative HCBCs into the subretinal space of severe combined immunodeficiency (SCID) mice. Two weeks after the transplantation, some of the transplanted cells expressed human nestin, human MAP2, human neuron specific enolase (NSE), beta-III tubulin and also rhodopsin. These results indicate that HCBCs can differentiate into RNCs and suggest that our new strategy could be used for the regeneration of retinal nerve cells in degenerative or dystrophic diseases.
视网膜变性和营养不良是发达国家失明的主要原因。据报道，人类脐带血细胞（HCBCs）在体外可分化为神经元样细胞。我们最近证明，小鼠和大鼠的骨髓细胞（BMCs）均可分化为视网膜神经细胞（RNCs）。在本研究中，我们展示了HCBCs在体内分化为RNCs的能力。我们将谱系阴性HCBCs移植到严重联合免疫缺陷（SCID）小鼠的视网膜下间隙。移植后两周，一些移植细胞表达人类巢蛋白、人类微管相关蛋白2（MAP2）、人类神经元特异性烯醇化酶（NSE）、β-III微管蛋白以及视紫红质。这些结果表明HCBCs可分化为RNCs，并提示我们的新策略可用于退行性或营养不良性疾病中视网膜神经细胞的再生。
