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人脐带间充质干细胞移植治疗视神经损伤大鼠的疗效。

Therapeutic potential of human umbilical cord-derived mesenchymal stem cells transplantation in rats with optic nerve injury.

机构信息

Department of Ophthalmology, Universiti Kebangsaan Malaysia Medical Centre, Melbourne, Australia.

Centre for Eye Research Australia, Royal Victorian Eye and Ear Hospital; Ophthalmology, Department of Surgery, University of Melbourne, Melbourne, Australia.

出版信息

Indian J Ophthalmol. 2022 Jan;70(1):201-209. doi: 10.4103/ijo.IJO_473_21.

DOI:10.4103/ijo.IJO_473_21
PMID:34937239
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8917541/
Abstract

PURPOSE

There are no effective treatments currently available for optic nerve transection injuries. Stem cell therapy represents a feasible future treatment option. This study investigated the therapeutic potential of human umbilical cord-derived mesenchymal stem cell (hUC-MSC) transplantation in rats with optic nerve injury.

METHODS

Sprague-Dawley (SD) rats were divided into three groups: a no-treatment control group (n = 6), balanced salt solution (BSS) treatment group (n = 6), and hUC-MSCs treatment group (n = 6). Visual functions were assessed by flash visual evoked potential (fVEP) at baseline, Week 3, and Week 6 after optic nerve crush injury. Right eyes were enucleated after 6 weeks for histology.

RESULTS

The fVEP showed shortened latency delay and increased amplitude in the hUC-MSCs treated group compared with control and BSS groups. Higher cellular density was detected in the hUC-MSC treated group compared with the BSS and control groups. Co-localized expression of STEM 121 and anti-S100B antibody was observed in areas of higher nuclear density, both in the central and peripheral regions.

CONCLUSION

Peribulbar transplantation of hUC-MSCs demonstrated cellular integration that can potentially preserve the optic nerve function with a significant shorter latency delay in fVEP and higher nuclear density on histology, and immunohistochemical studies observed cell migration particularly to the peripheral regions of the optic nerve.

摘要

目的

目前，对于视神经横断损伤，尚无有效的治疗方法。干细胞治疗代表了一种可行的未来治疗选择。本研究探讨了人脐带间充质干细胞（hUC-MSC）移植治疗视神经损伤大鼠的治疗潜力。

方法

将 Sprague-Dawley（SD）大鼠分为三组：无治疗对照组（n = 6）、平衡盐溶液（BSS）治疗组（n = 6）和 hUC-MSCs 治疗组（n = 6）。在视神经挤压伤后基线、第 3 周和第 6 周，通过闪光视觉诱发电位（fVEP）评估视觉功能。6 周后，右眼行组织学检查。

结果

fVEP 显示，与对照组和 BSS 组相比，hUC-MSCs 治疗组潜伏期延迟缩短，振幅增加。与 BSS 组和对照组相比，hUC-MSCs 治疗组细胞密度更高。在中央和周围区域，均观察到 STEM 121 和抗 S100B 抗体的共定位表达，在核密度较高的区域。

结论

球周注射 hUC-MSCs 具有细胞整合作用，能显著缩短 fVEP 的潜伏期延迟，提高组织学的核密度，并通过免疫组织化学研究观察到细胞向视神经的外周区域迁移，从而可能保留视神经功能。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1cb/8917541/65688c5016fc/IJO-70-201-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1cb/8917541/0c9281d7b4ec/IJO-70-201-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1cb/8917541/f2bc08660833/IJO-70-201-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1cb/8917541/0062ba8dce18/IJO-70-201-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1cb/8917541/5e8de42a0c62/IJO-70-201-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1cb/8917541/27c0e1739991/IJO-70-201-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1cb/8917541/a369850343c5/IJO-70-201-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1cb/8917541/65688c5016fc/IJO-70-201-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1cb/8917541/0c9281d7b4ec/IJO-70-201-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1cb/8917541/f2bc08660833/IJO-70-201-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1cb/8917541/0062ba8dce18/IJO-70-201-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1cb/8917541/5e8de42a0c62/IJO-70-201-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1cb/8917541/27c0e1739991/IJO-70-201-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1cb/8917541/a369850343c5/IJO-70-201-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a1cb/8917541/65688c5016fc/IJO-70-201-g007.jpg

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