Katsunuma Yu, Hanazumi Michihito, Fujisaki Hirokazu, Minato Hajime, Kataoka Yasushi, Sawada Takushi, Hashimoto Yasushi, Yonemochi Chisato
Scientific Feeds Research Center, Japan Scientific Feeds Association, Narita, Chiba, Japan.
J Gen Appl Microbiol. 2008 Feb;54(1):39-50. doi: 10.2323/jgam.54.39.
Seven hundred thirty-nine animal strains and 662 livestock-farmer strains, consisting of Escherichia coli and enterococci, were examined for their pulsed-field gel electrophoresis (PFGE) and antimicrobial-resistance patterns. Two hundred fifty-eight and 203 PFGE patterns were found among 739 animal strains isolated from animals comprising broilers, pigs and cattle, and 662 human strains isolated from livestock farmers, respectively, from 27 farms in Japan. These results demonstrated that the PFGE patterns found among E. coli and enterococci strains from animals and livestock-farmers were heterogeneous and considerably diverse. The strains having both the identical PFGE pattern and the same drug-resistance pattern were defined as a single clone in this study. Seven types of E. coli and enterococci clones were shared among animals within the same farms and between the different farms housing the same animal species. The 25 strains (3.4%) of 739 E. coli and enterococci animal strains belonged to these seven types of clones. Only three types of E. coli clones were shared among animals between the different farms housing different animal species, but no identical E. faecalis or E. faecium clones were found between different animal species farms. The 15 strains (2.0%) of 739 E. coli and enterococci animal strains belonged to these three types of clones. Additionally, the 11 strains (1.5%) of 739 E. coli and enterococci strains isolated from animals were identical clones to strains isolated from livestock farmers of the same farm. These results suggest that the transmission of animal clones to livestock farmers or vice versa is less common.
对739株动物菌株和662株养殖户菌株（包括大肠杆菌和肠球菌）进行了脉冲场凝胶电泳（PFGE）和抗菌药物耐药模式检测。从日本27个农场的肉鸡、猪和牛等动物中分离出739株动物菌株，从养殖户中分离出662株人类菌株，分别发现了258种和203种PFGE模式。这些结果表明，动物和养殖户来源的大肠杆菌和肠球菌菌株中的PFGE模式具有异质性且差异很大。在本研究中，具有相同PFGE模式和相同耐药模式的菌株被定义为单个克隆。在同一农场内的动物之间以及饲养相同动物种类的不同农场之间，共有7种大肠杆菌和肠球菌克隆。739株大肠杆菌和肠球菌动物菌株中的25株（3.4%）属于这7种克隆类型。在饲养不同动物种类的不同农场之间的动物中，仅共有3种大肠杆菌克隆，但在不同动物种类的农场之间未发现相同的粪肠球菌或屎肠球菌克隆。739株大肠杆菌和肠球菌动物菌株中的15株（2.0%）属于这3种克隆类型。此外，从动物中分离出的739株大肠杆菌和肠球菌菌株中的11株（1.5%）与同一农场的养殖户中分离出的菌株是相同克隆。这些结果表明，动物克隆向养殖户的传播或反之的情况不太常见。
