Jones Rosemary C, Capen Diane, Petersen Bodil, Jain Rakesh K, Duda Dan G
Department of Anesthesia and Critical Care, Massachusetts General Hospital and Harvard Medical School, 55 Fruit Street, Boston, Massachusetts 02114, USA.
Nat Protoc. 2008;3(3):378-87. doi: 10.1038/nprot.2007.536.
By providing insight into the cellular events of vascular injury and repair, experimental model systems seek to promote timely therapeutic strategies for human disease. The goal of many current studies of neovascularization is to identify cells critical to the process and their role in vascular channel assembly. We propose here a protocol to analyze, in an in vivo rodent model, vessel and capillary remodeling (reorganization and growth) in the injured lung. Sequential analyses of stages in the assembly of vascular structures, and of relevant cell types, provide further opportunities to study the molecular and cellular determinants of lung neovascularization.
通过深入了解血管损伤和修复的细胞事件，实验模型系统旨在推动针对人类疾病的及时治疗策略。当前许多关于新血管形成的研究目标是确定该过程中至关重要的细胞及其在血管通道组装中的作用。我们在此提出一种方案，用于在体内啮齿动物模型中分析受损肺中血管和毛细血管的重塑（重组和生长）。对血管结构组装阶段及相关细胞类型进行序贯分析，为研究肺新血管形成的分子和细胞决定因素提供了更多机会。
