Webb R H, Rogomentich F
Wellman Laboratories of Photomedicine, 40 Blossom Street, Boston, Massachusetts 02114, USA.
Appl Opt. 1999 Aug 1;38(22):4870-5. doi: 10.1364/ao.38.004870.
A confocal microscope that uses separate, noncollinear objective lenses for illumination and collection makes it possible to work 20 mm from a sample with a field of view of 2.25 mm across and still achieve lateral and axial resolutions below 5 microm. The design is expected to have application to in vivo confocal microscopy, allowing a field of view and section thickness similar to those used by pathologists in examining excised tissue.
一种共聚焦显微镜，它使用单独的、非共线的物镜进行照明和采集，使得在距离样本20毫米处工作成为可能，其视场直径为2.25毫米，并且横向和轴向分辨率仍能低于5微米。这种设计有望应用于体内共聚焦显微镜检查，从而获得与病理学家检查切除组织时所使用的类似的视场和切片厚度。
