Orlofsky A, Berger M S, Prystowsky M B
Department of Pathology, University of Pennsylvania, Philadelphia.
Cell Regul. 1991 May;2(5):403-12. doi: 10.1091/mbc.2.5.403.
Granulocyte/macrophage colony-stimulating factor (GM-CSF) specifically induces the growth of myeloid progenitors and their maturation into neutrophils and macrophages. We have identified a series of previously uncharacterized hematopoietic-specific mRNAs that are expressed in myelopoietic mouse bone marrow cultures stimulated by GM-CSF. One of these messages, C10, encodes a new member of the family of cytokine-like genes related to macrophage inflammatory protein-1 (MIP-1). Members of this family are all induced by one or more stimuli related to inflammation, wound repair, or immune response. In contrast, C10 mRNA showed little or no accumulation in response to such activating agents and was greatly reduced on activation of a T-cell line. On the other hand, C10 mRNA, unlike MIP-1, was acutely stimulated during the first day of bone marrow culture in GM-CSF, and it was also strongly elevated during the induction of neutrophilic differentiation of 32D cl3 cells by granulocyte colony-stimulating factor. The implications of this unusual expression pattern are discussed.
粒细胞/巨噬细胞集落刺激因子（GM-CSF）特异性诱导髓系祖细胞生长并使其成熟为中性粒细胞和巨噬细胞。我们鉴定出一系列以前未被表征的造血特异性mRNA，它们在GM-CSF刺激的小鼠骨髓造血培养物中表达。其中一个名为C10的信使RNA编码与巨噬细胞炎性蛋白-1（MIP-1）相关的细胞因子样基因家族的一个新成员。该家族成员均由一种或多种与炎症、伤口修复或免疫反应相关的刺激诱导产生。相比之下，C10 mRNA在受到此类激活剂刺激时几乎没有积累，在T细胞系激活时则大幅减少。另一方面，与MIP-1不同，C10 mRNA在GM-CSF刺激的骨髓培养的第一天受到急性刺激，并且在粒细胞集落刺激因子诱导32D cl3细胞向中性粒细胞分化的过程中也强烈升高。本文讨论了这种异常表达模式的意义。
