Hara Kanae, Fujisawa Kenji, Nagai Hirokazu, Takamaru Natsumi, Ohe Go, Tsuru Kanji, Ishikawa Kunio, Miyamoto Youji
Department of Oral Surgery, Institute of Biomedical Sciences, Tokushima University Graduate School, 3-18-15 Kuramotocho, Tokushima 770-8504, Japan.
Department of Biomaterials, Faculty of Dental Science, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
Materials (Basel). 2016 Aug 23;9(9):711. doi: 10.3390/ma9090711.
Carbonate apatite (CO₃Ap) foam has gained much attention in recent years because of its ability to rapidly replace bone. However, its mechanical strength is extremely low for clinical use. In this study, to understand the potential of gelatin-reinforced CO₃Ap foam for bone replacement, CO₃Ap foam was reinforced with gelatin and the resulting physical characteristics were evaluated. The mechanical strength increased significantly with the gelatin reinforcement. The compressive strength of gelatin-free CO₃Ap foam was 74 kPa whereas that of the gelatin-reinforced CO₃Ap foam, fabricated using 30 mass % gelatin solution, was approximately 3 MPa. Heat treatment for crosslinking gelatin had little effect on the mechanical strength of the foam. The gelatin-reinforced foam did not maintain its shape when immersed in a saline solution as this promoted swelling of the gelatin; however, in the same conditions, the heat-treated gelatin-reinforced foam proved to be stable. It is concluded, therefore, that heat treatment is the key to the fabrication of stable gelatin-reinforced CO₃Ap foam.
近年来，碳酸磷灰石（CO₃Ap）泡沫因其能够快速替代骨骼而备受关注。然而，其机械强度极低，无法用于临床。在本研究中，为了解明明胶增强CO₃Ap泡沫用于骨替代的潜力，用明胶增强CO₃Ap泡沫并评估其由此产生的物理特性。随着明胶增强，机械强度显著提高。不含明胶的CO₃Ap泡沫的抗压强度为74 kPa，而使用30质量%明胶溶液制备的明胶增强CO₃Ap泡沫的抗压强度约为3 MPa。用于明胶交联的热处理对泡沫的机械强度影响不大。当浸入盐溶液中时，明胶增强泡沫无法保持其形状，因为这会促进明胶膨胀；然而，在相同条件下，经热处理的明胶增强泡沫被证明是稳定的。因此可以得出结论，热处理是制备稳定的明胶增强CO₃Ap泡沫的关键。
