Pinkas H, Fisch B, Rozansky G, Felz C, Kessler-Icekson G, Krissi H, Nitke S, Ao A, Abir R
IVF Research Laboratory, Infertility and IVF Unit, Helen Schneider Hospital for Women, Rabin Medical Center, Beilinson Campus, Petach Tikva 49100, Israel.
Mol Hum Reprod. 2008 Apr;14(4):199-206. doi: 10.1093/molehr/gan011. Epub 2008 Mar 7.
There is no information regarding the presence of platelet-derived growth factors (PDGFs) and their receptors in human ovaries. The expression of PDGF-A, -B and their two receptors, PDGFR-alpha and -beta, was investigated in ovarian samples from women/girls and from human fetuses, at the protein and mRNA levels. The samples were prepared for immunohistochemical staining for PDGF-A and -B and their two receptors and in situ hybridization for the detection of the mRNA transcripts of the receptors. Total RNA was extracted from frozen ovarian samples, and the expression of PDGF-A and -B was investigated by reverse transcription-polymerase chain reaction. The proteins for PDGF-A and -B were detected in oocytes, and in granulosa cells (GC) of 50% of the follicles from women/girls. The proteins and mRNA transcripts for the two receptors were detected in oocytes (mRNA for PDGFR-beta only in 25% of the oocytes). PDGFR-alpha mRNA was expressed in GC of a minority of the samples from women/girls, whereas PDGFR-beta protein and mRNA were identified in over 50% of the GC from this source. PDGF-A and -B transcripts were identified in all the extracts. The presence of the receptors in GC suggests that PDGFs might be involved in the activation of primordial follicles.
目前尚无关于人卵巢中血小板衍生生长因子（PDGFs）及其受体存在情况的信息。我们在蛋白质和mRNA水平上，对来自成年女性/女孩以及人类胎儿的卵巢样本中PDGF - A、- B及其两种受体PDGFR - α和 - β的表达进行了研究。制备样本用于PDGF - A、- B及其两种受体的免疫组织化学染色以及用于检测受体mRNA转录本的原位杂交。从冷冻的卵巢样本中提取总RNA，并通过逆转录 - 聚合酶链反应研究PDGF - A和 - B的表达。在卵母细胞以及成年女性/女孩50%的卵泡的颗粒细胞（GC）中检测到了PDGF - A和 - B蛋白。在卵母细胞中检测到了两种受体的蛋白和mRNA转录本（仅25%的卵母细胞中有PDGFR - β的mRNA）。成年女性/女孩少数样本的颗粒细胞中表达了PDGFR - α mRNA，而来自该来源的超过50%的颗粒细胞中鉴定出了PDGFR - β蛋白和mRNA。在所有提取物中都鉴定出了PDGF - A和 - B转录本。颗粒细胞中存在这些受体表明PDGFs可能参与原始卵泡的激活。
