Infertility and IVF Unit, Helen Schneider Hospital for Women, Rabin Medical Center, Beilinson Hospital, Petach Tikva 49100, Israel.
Reprod Biomed Online. 2010 Jul;21(1):109-17. doi: 10.1016/j.rbmo.2010.03.011. Epub 2010 Mar 15.
The expression of growth-differentiating factor 9 (GDF9) has not been studied in ovaries from girls and human fetuses nor has its receptor transforming growth factor-beta1 receptor (TGFbetaR1) been investigated in ovaries of girls/women. The aim of this study was to fill these gaps. Ovarian samples were obtained from 16 human fetuses at 21-35 gestational weeks and from 34 girls/women aged 5-39years. These specimens were prepared for immunohistochemical staining of the GDF9 and TGFbetaR1 proteins. Reverse transcription polymerase chain reaction was used to detect GDF9 mRNA transcripts and in-situ hybridization to localize TGFbetaR1 mRNA transcripts. Positive staining for the GDF9 protein was identified in oocytes and granulosa cells in all samples tested. GDF9 mRNA transcripts were present in all samples. Protein expression of TGFbetaR1 was identified in granulosa cells in all samples. Oocyte staining was identified in samples from girls/women but in only one fetal sample. TGFbetaR1 mRNA transcripts were identified in granulosa cells and oocytes in 50% of the samples from fetuses aged over 22 gestational weeks and in samples from girls/women. The detection of GDF9 and TGFbetaR1 at both at the protein and mRNA levels suggests that GDF9 may have functions in human preantral follicles.
生长分化因子 9（GDF9）的表达尚未在女孩和人类胎儿的卵巢中进行研究，也未在女孩/妇女的卵巢中研究转化生长因子-β1 受体（TGFbetaR1）。本研究旨在填补这些空白。从 21-35 孕周的 16 个人类胎儿和 34 名 5-39 岁的女孩/妇女中获得卵巢样本。这些标本用于 GDF9 和 TGFbetaR1 蛋白的免疫组织化学染色。逆转录聚合酶链反应用于检测 GDF9 mRNA 转录本，原位杂交用于定位 TGFbetaR1 mRNA 转录本。在所有测试的样本中，均发现 GDF9 蛋白在卵母细胞和颗粒细胞中呈阳性染色。所有样本均存在 GDF9 mRNA 转录本。TGFbetaR1 蛋白在所有样本的颗粒细胞中表达。在女孩/妇女的样本中发现了卵母细胞染色，但在只有一个胎儿样本中发现了卵母细胞染色。在超过 22 孕周的胎儿样本和女孩/妇女的样本中，TGFbetaR1 mRNA 转录本在颗粒细胞和卵母细胞中均有发现。在蛋白质和 mRNA 水平上检测到 GDF9 和 TGFbetaR1 表明 GDF9 可能在人类窦前卵泡中有功能。
