Farimani Marzie, Heshmati Safoura, Poorolajal Jalal, Bahmanzadeh Maryam
Endometrium and Endometriosis Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.
Department of Genecology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
Mol Biol Rep. 2019 Apr;46(2):1611-1616. doi: 10.1007/s11033-019-04609-w. Epub 2019 Feb 5.
The prevalence of poor response to gonadotropin stimulation is approximately 9-24% in women undergoing in vitro fertilization. Interestingly, due to containing a variety of growth factors, platelet-rich plasma (PRP) can play an important role in tissue regeneration and healing. Thus, in this research, we aimed to investigate the intra-ovarian injection of PRP in women with poor ovarian response. To this goal, 23 poor responders constituted the study population, from among whom 19 women were enrolled. These patients underwent ovarian stimulation according to the Shanghai protocol. Immediately after the first follicular puncture, 2 mL of PRP was injected into each ovary. 1 day after the first puncture and PRP injection, the second stimulation was initiated. Then, oocyte retrieval was followed. About 2-3 months after the first cycle, the patients underwent another treatment with ovarian stimulation according to the Shanghai protocol and then, follicular puncture was performed. The mean numbers of oocytes before and after PRP injection were 0.64 and 2.1, respectively. Two patients experienced spontaneous conceptions. The third case achieved clinical pregnancy and delivered a healthy baby in June 2018. The results of this study appeared to be the first report on the effects of intra-ovarian PRP injection on the increase of ovarian responses, even on the spontaneous conceptions of women with poor ovarian response.
在接受体外受精的女性中，对促性腺激素刺激反应不良的发生率约为9%-24%。有趣的是，富含血小板血浆（PRP）由于含有多种生长因子，可在组织再生和愈合中发挥重要作用。因此，在本研究中，我们旨在探讨对卵巢反应不良的女性进行卵巢内注射PRP的效果。为此，23名反应不良者构成了研究人群，其中19名女性被纳入研究。这些患者按照上海方案进行卵巢刺激。在首次卵泡穿刺后，立即向每个卵巢注射2 mL PRP。在首次穿刺和注射PRP后1天，开始第二次刺激。然后进行卵母细胞采集。在第一个周期后约2-3个月，患者按照上海方案再次接受卵巢刺激治疗，然后进行卵泡穿刺。注射PRP前后卵母细胞的平均数量分别为0.64和2.1。两名患者自然受孕。第三例患者实现临床妊娠，并于2018年6月产下一名健康婴儿。本研究结果似乎是关于卵巢内注射PRP对增加卵巢反应，甚至对卵巢反应不良女性自然受孕影响的首份报告。
