Singh Shailendra, Mulchandani Ashok, Chen Wilfred
Department of Chemical and Environmental Engineering, University of California, Riverside, CA 92521.
Appl Environ Microbiol. 2008 May;74(9):2924-7. doi: 10.1128/AEM.02871-07. Epub 2008 Mar 7.
An arsenic-chelating metallothionein (fMT) from the arsenic-tolerant marine alga Fucus vesiculosus was expressed in Escherichia coli, resulting in 30- and 26-fold-higher As(III) and As(V) binding, respectively. Coexpression of the As(III)-specific transporter GlpF with fMT further improved arsenic accumulation and offered high selectivity toward As. Resting E. coli cells coexpressing fMT and GlpF completely removed trace amounts (35 ppb) of As(III) within 20 min, providing a promising technology for compliance with the As limit of 10 ppb newly recommended by the U.S. EPA.
从耐砷海洋藻类墨角藻中提取的一种砷螯合金属硫蛋白（fMT）在大肠杆菌中表达，结果显示其对As(III)和As(V)的结合能力分别提高了30倍和26倍。As(III)特异性转运蛋白GlpF与fMT共表达进一步提高了砷的积累，并对砷具有高选择性。共表达fMT和GlpF的静止大肠杆菌细胞在20分钟内完全去除了痕量（35 ppb）的As(III)，为符合美国环境保护局新推荐的10 ppb砷限量提供了一项有前景的技术。
