Shenzhen Prevention and Treatment Center for Occupational Diseases, Shenzhen, China.
School of Public Health, Guangdong Medical University, Dongguan, China.
World J Microbiol Biotechnol. 2024 May 6;40(6):192. doi: 10.1007/s11274-024-04001-2.
The global concern over arsenic contamination in water due to its natural occurrence and human activities has led to the development of innovative solutions for its detection and remediation. Microbial metabolism and mobilization play crucial roles in the global cycle of arsenic. Many microbial arsenic-resistance systems, especially the ars operons, prevalent in bacterial plasmids and genomes, play vital roles in arsenic resistance and are utilized as templates for designing synthetic bacteria. This review novelty focuses on the use of these tailored bacteria, engineered with ars operons, for arsenic biosensing and bioremediation. We discuss the advantages and disadvantages of using synthetic bacteria in arsenic pollution treatment. We highlight the importance of genetic circuit design, reporter development, and chassis cell optimization to improve biosensors' performance. Bacterial arsenic resistances involving several processes, such as uptake, transformation, and methylation, engineered in customized bacteria have been summarized for arsenic bioaccumulation, detoxification, and biosorption. In this review, we present recent insights on the use of synthetic bacteria designed with ars operons for developing tailored bacteria for controlling arsenic pollution, offering a promising avenue for future research and application in environmental protection.
由于砷的自然存在和人类活动，全球对水中砷污染的关注导致了针对其检测和修复的创新解决方案的发展。微生物代谢和迁移在砷的全球循环中起着至关重要的作用。许多微生物砷抗性系统，特别是在细菌质粒和基因组中普遍存在的 ars 操纵子，在砷抗性中起着重要作用，并被用作设计合成细菌的模板。本综述重点介绍了使用带有 ars 操纵子的定制细菌进行砷生物传感和生物修复。我们讨论了在砷污染处理中使用合成细菌的优缺点。我们强调了遗传电路设计、报告基因开发和底盘细胞优化的重要性，以提高生物传感器的性能。我们总结了针对砷生物积累、解毒和生物吸附的定制细菌中涉及多个过程（如摄取、转化和甲基化）的细菌砷抗性。在本综述中，我们介绍了使用带有 ars 操纵子设计的合成细菌来开发定制细菌以控制砷污染的最新见解，为未来的环境保护研究和应用提供了一个有前途的途径。
