Shyur Lie-Fen, Huang Chi-Chang, Lo Chiu-Ping, Chiu Chih-Yang, Chen Yi-Ping, Wang Sheng-Yang, Chang Shang-Tzen
Agricultural Biotechnology Research Center, Academia Sinica, Taipei 115, Taiwan, ROC.
Phytochemistry. 2008 Apr;69(6):1348-58. doi: 10.1016/j.phytochem.2008.01.017. Epub 2008 Mar 6.
Cryptomeria japonica is an important plantation conifer tree in Asia. This study aimed to characterize the anti-inflammatory and hepatoprotective activities of the phytocompounds from C. japonica wood on LPS- or TPA-induced activation of proinflammatory mediators and CCl(4)-induced acute liver injury in mice. A CJH7-2 fraction was purified from C. japonica extracts following bioactivity-guided fractionation, and it exhibited significant activities on inhibition of NO production and iNOS expression as well as up-regulating HO-1 expression in LPS-stimulated macrophages. CJH7-2 also potently inhibits COX-2 enzymatic activity (IC(50)=5 microg/mL) and TPA-induced COX-2 protein expression in mouse skin (1mg/200 microL/site). CJH7-2 (10 mg/kg BW) can prevent CCl(4)-induced liver injury and aminotransferases activities in mice. Chemical fingerprinting analysis showed that terpenes are the major bioactive compounds in the CJH7-2 fraction. This is the first study to demonstrate that chemical constituents from the wood extract of C. japonica possess anti-inflammatory activities in vitro and in vivo that may play a role in hepatoprotection.
日本柳杉是亚洲一种重要的人工针叶树。本研究旨在表征日本柳杉木材中的植物化合物对脂多糖（LPS）或佛波酯（TPA）诱导的促炎介质激活以及四氯化碳（CCl₄）诱导的小鼠急性肝损伤的抗炎和保肝活性。通过生物活性导向分级分离从日本柳杉提取物中纯化得到一个CJH7 - 2组分，它在抑制LPS刺激的巨噬细胞中一氧化氮（NO）产生和诱导型一氧化氮合酶（iNOS）表达以及上调血红素加氧酶 - 1（HO - 1）表达方面表现出显著活性。CJH7 - 2还能有效抑制环氧合酶 - 2（COX - 2）的酶活性（IC₅₀ = 5μg/mL）以及TPA诱导的小鼠皮肤中COX - 2蛋白表达（1mg/200μL/部位）。CJH7 - 2（10mg/kg体重）可预防CCl₄诱导的小鼠肝损伤和转氨酶活性。化学指纹图谱分析表明，萜类化合物是CJH7 - 2组分中的主要生物活性化合物。这是第一项证明日本柳杉木材提取物中的化学成分在体外和体内具有抗炎活性且可能在肝脏保护中发挥作用的研究。
