Koo Hyun Jung, Yoon Weon-Jong, Sohn Eun-Hwa, Ham Young-Min, Jang Seon-A, Kwon Jung-Eun, Jeong Yong Joon, Kwak Jong Hwan, Sohn Eunsoo, Park Soo-Young, Jang Ki-Hyo, Namkoong Seung, Han Hyo-Sang, Jung Yong-Hwan, Kang Se Chan
Department of Life Science, Gachon University, 1342 Seongnamdaero, Seongnam 461-701, Republic of Korea.
Jeju Biodiversity Research Institute, Jeju Technopark, Seogwipo 697-943, Republic of Korea.
Int Immunopharmacol. 2014 Sep;22(1):84-97. doi: 10.1016/j.intimp.2014.06.007. Epub 2014 Jun 23.
Fruits of the Litsea family of trees and shrubs contain biologically active compounds, some of which have been used as natural nutrients and flavoring agents in food. In this study, we identified novel anti-nociceptive effects of the 30% ethanol extract, the CH(2)Cl(2) fraction and the associated active components (Hamabiwalactone A and B) from Litsea japonica fruit by using in vivo peripheral and central nervous pain models. In addition, we compared the anti-inflammatory effects of several fractions from L. japonica fruit extracts using lipopolysaccharide (LPS)-stimulated Raw264.7 cells. The CH(2)Cl(2) fraction of L. japonica fruit (LJM) had an optimal combination of anti-inflammatory effects and low cytotoxicity. Dose response studies were performed to determine the inhibitory effects of LJM on the pro-inflammatory enzymes, COX-2/PGE(2) and NO/iNOS, and pro-inflammatory cytokines, IL-1β, IL-6, and TNF-α. Molecular profiling revealed that LJM exerts anti-inflammatory effects through inhibition of NF-κB and JNK/p38 MAPK signaling in LPS-induced macrophages. This study suggests that CH2Cl2 fraction of L. japonica fruit and its bioactive components are potential candidates as anti-inflammatory and analgesic agents (painkillers) for the treatment of inflammatory diseases.
樟科树木和灌木的果实含有生物活性化合物，其中一些已被用作食品中的天然营养成分和调味剂。在本研究中，我们通过体内外周和中枢神经疼痛模型，确定了日本山鸡椒果实30%乙醇提取物、二氯甲烷馏分及其相关活性成分（滨比瓦内酯A和B）的新型抗伤害感受作用。此外，我们使用脂多糖（LPS）刺激的Raw264.7细胞，比较了日本山鸡椒果实提取物几个馏分的抗炎作用。日本山鸡椒果实的二氯甲烷馏分（LJM）具有抗炎作用和低细胞毒性的最佳组合。进行了剂量反应研究，以确定LJM对促炎酶COX-2/PGE2和NO/iNOS以及促炎细胞因子IL-1β、IL-6和TNF-α的抑制作用。分子分析表明，LJM通过抑制LPS诱导的巨噬细胞中的NF-κB和JNK/p38 MAPK信号传导发挥抗炎作用。本研究表明，日本山鸡椒果实的二氯甲烷馏分及其生物活性成分是治疗炎症性疾病的抗炎和镇痛剂（止痛药）的潜在候选物。
