Agricultural Biotechnology Research Center, Academia Sinica, Nankang, Taipei, Taiwan, ROC.
Eur J Appl Physiol. 2010 Feb;108(3):557-66. doi: 10.1007/s00421-009-1247-7. Epub 2009 Oct 29.
Exhaustive exercise and endurance exercise training modify the physiological status of the body differently. The present study aimed to evaluate the alteration in biochemical composition with exhaustive and endurance exercises in rats using metabolomics strategy. The metabolite profile of liver tissue was investigated on gas chromatography-mass spectrometry (GC-MS). Data further underwent partial least-squares-discriminant analysis (PLS-DA) to compare the effects on metabolites in sedentary control, exhaustively exercised and endurance trained rats. GC-MS detected 115 highly reproducible peaks in chromatograms from individual liver tissue extracts, and we identified 55 of them. The three groups showed significant differences in metabolic profile. Changes in liver metabolism involved metabolites such as amino acids, fatty acids, organic acids, and carbohydrates. Endurance training elevated the greater rate of tricarboxylic acid cycle and antioxidant activity, and exhaustive exercise led to accumulated urea markers and an inflammation response in liver. In addition, GC-MS-based metabolomic analysis is a promising tool to investigate a pathological status with different exercise programs.
剧烈运动和耐力运动训练以不同的方式改变身体的生理状态。本研究旨在使用代谢组学策略评估大鼠在剧烈运动和耐力运动训练后生化成分的变化。采用气相色谱-质谱联用技术（GC-MS）对肝组织的代谢物谱进行了研究。数据进一步经过偏最小二乘判别分析（PLS-DA），以比较久坐对照组、剧烈运动组和耐力训练组的代谢物影响。GC-MS 在个体肝组织提取物的色谱图中检测到 115 个高度重现性的峰，我们鉴定出其中的 55 个。三组的代谢谱存在显著差异。肝代谢的变化涉及氨基酸、脂肪酸、有机酸和碳水化合物等代谢物。耐力训练提高了三羧酸循环和抗氧化活性的更高速率，而剧烈运动导致尿素标志物的积累和肝脏炎症反应。此外，基于 GC-MS 的代谢组学分析是研究不同运动方案下病理状态的有前途的工具。
