School of Chemistry, Institute of Science, Suranaree University of Technology (SUT), Nakhon Ratchasima, 30000, Thailand.
School of Biomolecular Science and Engineering (BSE), Vidyasirimedhi Institute of Science and Technology (VISTEC), Wangchan Valley, Rayong, 21210, Thailand.
Mikrochim Acta. 2019 Aug 13;186(9):616. doi: 10.1007/s00604-019-3740-y.
Glucose oxidase was soaked into a porous carbon nanotube film coating on a platinum disk electrode, then trapped beneath a topcoat of electrodeposition paint. The resulting sensors, operated at a potential of +0.6 V (vs. Ag/AgCl), produced a glucose signal that was linear up to 40 mM, with a 50 μM detection limit. Signal stability over >100 h of continuous operation in a flow cell showed the remarkable functional durability of the biosensor, and confirmed that the electropaint coating effectively prevented loss of the enzyme. This performance is deemed to derive from the minimalistic immobilization layer design and the prevention of protein leakage. The immobilization method has a potentially wide scope, in that it may also be applicable in other types of enzymatic biosensor. Graphical abstract Illustration of an enzyme biosensor design that uses glucose oxidase in bare carbon nanotube electrode modifications with electropaint topcoat for amperometric glucose quantification. Immobilization matrix supplementation with extra functional (nano-) materials was unnecessary for high-quality and stable analysis performance.
葡萄糖氧化酶被浸泡在多孔碳纳米管薄膜涂层中，该涂层涂覆在铂盘电极上，然后被电沉积漆的顶层涂层覆盖。所得传感器在+0.6 V（相对于 Ag/AgCl）的电势下工作，产生的葡萄糖信号在 40 mM 以内呈线性，检测限为 50 μM。在流动池中连续运行超过 100 小时的信号稳定性表明，该生物传感器具有出色的功能耐用性，并证实电涂覆层有效地防止了酶的损失。这种性能被认为源于最小化的固定化层设计和蛋白质泄漏的防止。该固定化方法具有广泛的应用潜力，因为它也可能适用于其他类型的酶生物传感器。示意图说明使用裸碳纳米管电极修饰中的葡萄糖氧化酶的酶生物传感器设计，并用电涂覆层顶层涂层进行安培葡萄糖定量。对于高质量和稳定的分析性能，固定化基质中补充额外的功能（纳米）材料是不必要的。
