Li Yu-Jie, Yang Qing, Wen Xiao-Gang, Zhu Xiao-Xing
Institute of Chinese Materia Medica, China Academy of Chinese Medical Science, Beijing 100700, China.
Zhongguo Zhong Yao Za Zhi. 2007 Dec;32(23):2500-4.
To analysis and evaluate the inflammatory reaction of atherosclerosis model in rabbits.
A model of atherosclerosis in rabbits was estabalished by injury with balloon and high cholesterol diet to observe the dynamic change of serum inflammation markers c-reactive protein, interleukin-1beta and tumor necrosis factor-a, and the relationship between severity of AS lesion and those change.
(1) The levels of TNF-alpha at 4 time points: 1, 6, 10 weeks and 10 weeks + 1 day after balloon injury increased 1.6-fold, 2.2-fold, 4-fold, 2-fold over concurrent control, respectively (P < 0.05, P < 0.01, P < 0.01, P < 0.01), but no significant changes occurred during the observation. (2) At the end of the 6, 10 weeks and 10 weeks + 1 day, the levels of CRP were increased 3.5-fold, 3.6-fold, 3.0-fold than concurrent control (P < 0.01, P < 0.01, P < 0.01), respectively. The levels were increased 2.4-fold at the end of the 6 weeks and 4.1-fold at the end of the 10 weeks and reached the peak compared with the point of 1 week. (3) TNF-alpha and CRP showed a significant positive correlation with the MIT (correlation coefficients were found to be 0.61, 0.64, P < 0.01). (4) At the end of the 1, 6, 10 weeks, the model was provided with general pathological characteristics and the inflammatory reaction of earlier inflammatory reaction phase, fatty streak plaques phase, fibrous plaque phase respectively.
The model was provided with early and medial phase typical feature of atherosclerotic lesion, and showed a significant positive correlation with the inflammatory factor expression. At the end of the 6 weeks, the formation of fatty streak plaques and obvious inflammatory reaction could be satisfied for the interference in forming process of AS from dimension of inflammatory and screening assays of drugs.
分析和评估兔动脉粥样硬化模型的炎症反应。
通过球囊损伤和高胆固醇饮食建立兔动脉粥样硬化模型，观察血清炎症标志物c反应蛋白、白细胞介素-1β和肿瘤坏死因子-α的动态变化，以及动脉粥样硬化病变严重程度与这些变化之间的关系。
（1）球囊损伤后1周、6周、10周及10周 + 1天这4个时间点的肿瘤坏死因子-α水平分别比同期对照组升高1.6倍、2.2倍、4倍、2倍（P < 0.05，P < 0.01，P < 0.01，P < 0.01），但在观察期间无显著变化。（2）在6周、10周及10周 + 1天结束时，c反应蛋白水平分别比同期对照组升高3.5倍、3.6倍、3.0倍（P < 0.01，P < 0.01，P < 0.01）。与1周时相比，6周结束时升高2.4倍，10周结束时升高4.1倍并达到峰值。（3）肿瘤坏死因子-α和c反应蛋白与内膜中层厚度呈显著正相关（相关系数分别为0.61、0.64，P < 0.01）。（4）在1周、6周、10周结束时，模型分别具有早期炎症反应期、脂纹斑块期、纤维斑块期的一般病理特征和炎症反应。
该模型具有动脉粥样硬化病变的早期和中期典型特征，且与炎症因子表达呈显著正相关。在6周结束时，脂纹斑块的形成和明显的炎症反应可满足从炎症维度和药物筛选试验对动脉粥样硬化形成过程进行干预的要求。
