Li Jun, Zhang An, Chen Xiaoqiu, Ren Zhong, Pan Zimin
Department of Otorhinolaryngology, Affiliated Shengjing Hospital, China Medical University, Shenyang, 110004, China.
Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2007 Dec;21(24):1109-13.
To study the expression and prognostic significance of cyclooxygenase-2 (Cox-2) in supraglottic laryngeal squamous cell carcinomas (SGLSCC) and identify the relationship between Cox-2 and angiogenesis and the roles of Cox-2 in SGLSCC as a biological marker.
Eighty-eight primary SGLSCC patients received surgical treatment were studied by immunohistochemical staining, and reverse transcription-polymerase chain reaction (RT-PCR) technique.
The percentage of Cox-2-positive cells was 94.3% (83/88) in SGLSCC whereas there was no immunostaining in the all cells of normal mucosa of paracarcinoma. Cox-2 expression was higher in well-differentiated tumors compared with poorly-differentiated SGLSCC. The relative concentration of Cox-2 mRNA was 141.871 +/- 20.5435 in SGLSCC and 17.031 +/- 2.2597 in normal paracarcinoma mucosa (P < 0.01). It was significantly higher in SGLSCC than in normal paracarcinoma mucosa. In SGLSCC, only pathological grading and the percentage of Cox-2-positive cells had significant correlation (P < 0.01). And not only the percentage of Cox-2-positive cells but also Cox-2 intensity had significant correlation with microvessel density (MVD) (P < 0.01). Kaplan-Meier survival analysis showed there had significant relationship between Cox-2 intensity and cumulative survival rate of SGLSCC patients (P < 0.05). But the percentage of Cox-2-positive cells was different (P > 0.05). Cox's regression analysis indicated that Cox-2 intensity were significantly independent prognostic factors (P < 0.01).
Cox-2 expression maybe relate to the carcinogenesis and progress in tumors especially in well-differentiated ones; The changes of Cox-2 expression are synchronous with MVD. Cox-2 intensity is a significantly independent prognostic factor. So Cox-2 may be an effective target of prevention, therapy and prognostic evaluation for laryngeal carcinoma and other head and neck squamous cell carcinomas (HNSCC).
研究环氧化酶-2（Cox-2）在声门上型喉鳞状细胞癌（SGLSCC）中的表达及其预后意义，明确Cox-2与血管生成之间的关系以及Cox-2作为生物学标志物在SGLSCC中的作用。
采用免疫组织化学染色及逆转录-聚合酶链反应（RT-PCR）技术，对88例接受手术治疗的原发性SGLSCC患者进行研究。
SGLSCC中Cox-2阳性细胞百分比为94.3%（83/88），而癌旁正常黏膜所有细胞均无免疫染色。与低分化SGLSCC相比，高分化肿瘤中Cox-2表达更高。SGLSCC中Cox-2 mRNA的相对浓度为141.871±20.5435，癌旁正常黏膜为17.031±2.2597（P<0.01）。SGLSCC中的表达显著高于癌旁正常黏膜。在SGLSCC中，仅病理分级与Cox-2阳性细胞百分比具有显著相关性（P<0.01）。Cox-2阳性细胞百分比及Cox-2强度均与微血管密度（MVD）具有显著相关性（P<0.01）。Kaplan-Meier生存分析显示，Cox-2强度与SGLSCC患者的累积生存率具有显著关系（P<0.05）。但Cox-2阳性细胞百分比差异无统计学意义（P>0.05）。Cox回归分析表明，Cox-2强度是显著的独立预后因素（P<0.01）。
Cox-2表达可能与肿瘤尤其是高分化肿瘤的发生发展有关；Cox-2表达变化与MVD同步。Cox-2强度是显著的独立预后因素。因此，Cox-2可能是喉癌及其他头颈部鳞状细胞癌（HNSCC）预防、治疗及预后评估的有效靶点。
