Wang Cheng-yuan, Xiao Shui-fang, Li Xue-pei, Sun Yan-tian
Department of Otorhinolaryngology Head and Neck Surgery, Peking University First Hospital, Beijing 100034, China.
Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2005 Aug;40(8):582-6.
To observe the expression of adrenomedullin (AM) in the patients with laryngeal carcinoma.
Two-step immunohistochemistry method was used to examine the expression of AM in the patients with laryngeal carcinoma. Radioimmunoassay was applied to determine the concentration of AM in the laryngeal carcinoma tissues, adjacent laryngeal mucosa of carcinoma tissues and in the plasma of patients and controls.
Positive stainings for AM were found in all 21 specimen examined,distributed mainly in the cytoplasm of the laryngeal carcinoma cells. Positive stainings were more stronger in the circumference than in the center of tumor tissue for the highly and moderately differentiated tumors. While the stainings were distributed homogeneously for poorly and moderately differentiated tumors. The concentration of AM in the laryngeal carcinoma tissues (n = 44) and the adjacent mucosa (n = 44) were (49.67 +/- 28.33) pg/ml and (14.71 +/- 7.17) pg/ml (x +/- s) respectively and laryngeal tumor showed much higher concentration of AM than the adjacent mucosa (u = 135.00, P < 0.01). The concentration of AM in patients with laryngeal carcinoma of T2, T3 and T4 stage were (31.52 +/- 15.22), (56.63 +/- 18.51) and (96.12 +/- 18.22) pg/ml (x + s) respectively,and there were statistically significant difference among them. In the N stage, patients with higher stages were found to express significantly higher AM concentration, but there was not statistically significant difference between NO stage and N1 stage. In the M stage,patients with M1 stage were found to express significantly higher AM concentration (u = 31.00, P < 0.01). But there was not statistically significant difference between AM plasma concentration of laryngeal carcinoma patients and that of healthy controls.
The results suggested that high expression of AM in tissues of laryngeal carcinoma was related with the TNM stage of laryngeal carcinoma, AM may play an important role in the development of the laryngeal neoplasma.
观察肾上腺髓质素（AM）在喉癌患者中的表达情况。
采用两步免疫组化法检测喉癌患者中AM的表达。应用放射免疫分析法测定喉癌组织、癌旁喉黏膜组织以及患者和对照组血浆中AM的浓度。
在所检测的21例标本中均发现AM阳性染色，主要分布于喉癌细胞的细胞质中。高分化和中分化肿瘤组织周边的阳性染色比中心更强。而低分化和未分化肿瘤组织中的染色分布均匀。喉癌组织（n = 44）和癌旁黏膜（n = 44）中AM的浓度分别为（49.67±28.33）pg/ml和（14.71±7.17）pg/ml（x±s），喉癌组织中AM的浓度明显高于癌旁黏膜（u = 135.00，P < 0.01）。T2、T3和T4期喉癌患者血浆中AM的浓度分别为（31.52±15.22）、（56.63±18.51）和（96.12±18.22）pg/ml（x + s），差异有统计学意义。在N分期中，分期较高的患者AM浓度明显升高，但NO期和N1期之间差异无统计学意义。在M分期中，M1期患者AM浓度明显升高（u = 31.00，P < 0.01）。但喉癌患者血浆中AM浓度与健康对照组之间差异无统计学意义。
结果表明，喉癌组织中AM的高表达与喉癌的TNM分期有关，AM可能在喉肿瘤的发生发展中起重要作用。
