一种来自果蝇的进化保守细胞周期蛋白同源物拯救了缺乏G1期细胞周期蛋白的酵母。
An evolutionarily conserved cyclin homolog from Drosophila rescues yeast deficient in G1 cyclins.
作者信息
Léopold P, O'Farrell P H
机构信息
Department of Biochemistry and Biophysics, University of California, San Francisco 94143.
出版信息
Cell. 1991 Sep 20;66(6):1207-16. doi: 10.1016/0092-8674(91)90043-x.
Abstract
We have isolated two Drosophila cDNA clones that rescue Saccharomyces cerevisiae deficient in CLN functions. One of these clones is the Drosophila homolog of the cdc2 gene. The second encodes a distant and new member of the cyclin family of proteins, cyclin C. It is highly homologous (72% identity) to a human clone isolated in a similar screen. Yeast cells rescued by a plasmid constitutively expressing this Drosophila cyclin C are unusually small, consistent with an unregulated high level of G1 cyclin function. Sequence comparisons identified regions conserved among the more distantly related cyclins. Based on these conserved elements, we identified homology between cyclins and the ras oncogene.
摘要
我们分离出了两个能拯救缺乏CLN功能的酿酒酵母的果蝇cDNA克隆。其中一个克隆是cdc2基因的果蝇同源物。另一个克隆编码一种细胞周期蛋白家族中关系较远的新成员——细胞周期蛋白C。它与在类似筛选中分离出的一个人类克隆高度同源（同一性为72%）。用组成型表达这种果蝇细胞周期蛋白C的质粒拯救的酵母细胞异常小，这与G1细胞周期蛋白功能不受调控的高水平一致。序列比较确定了在关系较远的细胞周期蛋白中保守的区域。基于这些保守元件，我们确定了细胞周期蛋白与ras癌基因之间的同源性。
相似文献
1
An evolutionarily conserved cyclin homolog from Drosophila rescues yeast deficient in G1 cyclins.一种来自果蝇的进化保守细胞周期蛋白同源物拯救了缺乏G1期细胞周期蛋白的酵母。
Cell. 1991 Sep 20;66(6):1207-16. doi: 10.1016/0092-8674(91)90043-x.
2
Isolation of three novel human cyclins by rescue of G1 cyclin (Cln) function in yeast.通过拯救酵母中的G1细胞周期蛋白（Cln）功能分离出三种新型人类细胞周期蛋白。
Cell. 1991 Sep 20;66(6):1197-206. doi: 10.1016/0092-8674(91)90042-w.
3
Human cyclin E, a new cyclin that interacts with two members of the CDC2 gene family.
Cell. 1991 Sep 20;66(6):1217-28. doi: 10.1016/0092-8674(91)90044-y.
4
A novel cyclin gene from Drosophila complements CLN function in yeast.
Genes Dev. 1991 Dec;5(12A):2166-75. doi: 10.1101/gad.5.12a.2166.
5
Human cyclin K, a novel RNA polymerase II-associated cyclin possessing both carboxy-terminal domain kinase and Cdk-activating kinase activity.人细胞周期蛋白K，一种新型的与RNA聚合酶II相关的细胞周期蛋白，兼具羧基末端结构域激酶和细胞周期蛋白依赖性激酶激活激酶活性。
Mol Cell Biol. 1998 Jul;18(7):4291-300. doi: 10.1128/MCB.18.7.4291.
6
A new human p34 protein kinase, CDK2, identified by complementation of a cdc28 mutation in Saccharomyces cerevisiae, is a homolog of Xenopus Eg1.
EMBO J. 1991 Sep;10(9):2653-9. doi: 10.1002/j.1460-2075.1991.tb07808.x.
7
CLB5: a novel B cyclin from budding yeast with a role in S phase.CLB5：一种来自芽殖酵母的新型B型细胞周期蛋白，在S期发挥作用。
Genes Dev. 1992 Sep;6(9):1695-706. doi: 10.1101/gad.6.9.1695.
8
Dhr6, a Drosophila homolog of the yeast DNA-repair gene RAD6.Dhr6，酵母DNA修复基因RAD6的果蝇同源物。
Proc Natl Acad Sci U S A. 1991 May 1;88(9):3832-6. doi: 10.1073/pnas.88.9.3832.
9
A Drosophila G1-specific cyclin E homolog exhibits different modes of expression during embryogenesis.一种果蝇G1期特异性细胞周期蛋白E同源物在胚胎发育过程中表现出不同的表达模式。
Development. 1993 Nov;119(3):673-90. doi: 10.1242/dev.119.3.673.
10
Molecular cloning and analysis of CDC28 and cyclin homologues from the human fungal pathogen Candida albicans.来自人类真菌病原体白色念珠菌的CDC28和细胞周期蛋白同源物的分子克隆与分析。
Mol Gen Genet. 1994 Dec 15;245(6):716-23. doi: 10.1007/BF00297278.
引用本文的文献
1
Cdk8/CDK19 promotes mitochondrial fission through Drp1 phosphorylation and can phenotypically suppress pink1 deficiency in Drosophila.Cdk8/CDK19 通过磷酸化 Drp1 促进线粒体裂变，并在果蝇中表型上抑制 pink1 缺失。
Nat Commun. 2024 Apr 18;15(1):3326. doi: 10.1038/s41467-024-47623-8.
2
Exploitation of the Mediator complex by viruses.病毒对中介体复合物的利用。
PLoS Pathog. 2022 Apr 21;18(4):e1010422. doi: 10.1371/journal.ppat.1010422. eCollection 2022 Apr.
3
Cyclin C: The Story of a Non-Cycling Cyclin.细胞周期蛋白C：一个非循环细胞周期蛋白的故事。
Biology (Basel). 2019 Jan 4;8(1):3. doi: 10.3390/biology8010003.
4
Cyclin C directly stimulates Drp1 GTP affinity to mediate stress-induced mitochondrial hyperfission.周期蛋白 C 直接刺激 Drp1 GTP 亲和力以介导应激诱导的线粒体过度分裂。
Mol Biol Cell. 2019 Feb 1;30(3):302-311. doi: 10.1091/mbc.E18-07-0463. Epub 2018 Dec 5.
5
Cyclin C influences the timing of mitosis in fission yeast.细胞周期蛋白C影响裂殖酵母有丝分裂的时间。
Mol Biol Cell. 2017 Jul 1;28(13):1738-1744. doi: 10.1091/mbc.E16-11-0787. Epub 2017 May 17.
6
Degradation of the Mitotic Cyclin Clb3 Is not Required for Mitotic Exit but Is Necessary for G1 Cyclin Control of the Succeeding Cell Cycle.有丝分裂周期蛋白Clb3的降解对于有丝分裂退出并非必需，但对于后续细胞周期的G1期周期蛋白控制是必要的。
Genetics. 2016 Dec;204(4):1479-1494. doi: 10.1534/genetics.116.194837. Epub 2016 Oct 28.
7
CDK8-Cyclin C Mediates Nutritional Regulation of Developmental Transitions through the Ecdysone Receptor in Drosophila.CDK8 - 细胞周期蛋白C通过果蝇中的蜕皮激素受体介导发育转变的营养调节。
PLoS Biol. 2015 Jul 29;13(7):e1002207. doi: 10.1371/journal.pbio.1002207. eCollection 2015 Jul.
8
Cyclin C is a haploinsufficient tumour suppressor.细胞周期蛋白C是一种单倍剂量不足的肿瘤抑制因子。
Nat Cell Biol. 2014 Nov;16(11):1080-91. doi: 10.1038/ncb3046. Epub 2014 Oct 26.
9
Cyclins and cell cycle control in cancer and disease.细胞周期蛋白与癌症及疾病中的细胞周期调控
Genes Cancer. 2012 Nov;3(11-12):649-57. doi: 10.1177/1947601913479022.
10
Orphan kinases turn eccentric: a new class of cyclin Y-activated, membrane-targeted CDKs.孤儿激酶变得古怪：一类新型的细胞周期蛋白 Y 激活的、膜靶向的 CDK。
Cell Cycle. 2012 Oct 15;11(20):3758-68. doi: 10.4161/cc.21592. Epub 2012 Aug 16.
本文引用的文献
1
Gene required in G1 for commitment to cell cycle and in G2 for control of mitosis in fission yeast.在裂殖酵母中，该基因在G1期对细胞周期进程是必需的，在G2期对有丝分裂的控制是必需的。
Nature. 1981 Aug 6;292(5823):558-60. doi: 10.1038/292558a0.
2
A bifunctional gene product involved in two phases of the yeast cell cycle.一种参与酵母细胞周期两个阶段的双功能基因产物。
Nature. 1982 Jul 22;298(5872):391-3. doi: 10.1038/298391a0.
3
Transformation of intact yeast cells treated with alkali cations.经碱金属阳离子处理的完整酵母细胞的转化
J Bacteriol. 1983 Jan;153(1):163-8. doi: 10.1128/jb.153.1.163-168.1983.
4
One-step gene disruption in yeast.酵母中的一步基因破坏
Methods Enzymol. 1983;101:202-11. doi: 10.1016/0076-6879(83)01015-0.
5
Protein kinase activity associated with the product of the yeast cell division cycle gene CDC28.与酵母细胞分裂周期基因CDC28的产物相关的蛋白激酶活性。
Proc Natl Acad Sci U S A. 1985 Jun;82(12):4055-9. doi: 10.1073/pnas.82.12.4055.
6
Comparison of the consensus sequence flanking translational start sites in Drosophila and vertebrates.果蝇和脊椎动物翻译起始位点侧翼共有序列的比较。
Nucleic Acids Res. 1987 Feb 25;15(4):1353-61. doi: 10.1093/nar/15.4.1353.
7
Unidirectional digestion with exonuclease III in DNA sequence analysis.DNA序列分析中用核酸外切酶III进行单向消化。
Methods Enzymol. 1987;155:156-65. doi: 10.1016/0076-6879(87)55014-5.
8
A ten-minute DNA preparation from yeast efficiently releases autonomous plasmids for transformation of Escherichia coli.从酵母中制备十分钟的DNA可有效释放自主质粒用于转化大肠杆菌。
Gene. 1987;57(2-3):267-72. doi: 10.1016/0378-1119(87)90131-4.
9
Transformation of yeast spheroplasts without cell fusion.无细胞融合的酵母原生质体转化
Anal Biochem. 1987 Jun;163(2):391-7. doi: 10.1016/0003-2697(87)90240-5.
10
DAF1, a mutant gene affecting size control, pheromone arrest, and cell cycle kinetics of Saccharomyces cerevisiae.DAF1，一个影响酿酒酵母大小控制、信息素停滞及细胞周期动力学的突变基因。
Mol Cell Biol. 1988 Nov;8(11):4675-84. doi: 10.1128/mcb.8.11.4675-4684.1988.
Zotero 插件