Léopold P, O'Farrell P H
Department of Biochemistry and Biophysics, University of California, San Francisco 94143.
Cell. 1991 Sep 20;66(6):1207-16. doi: 10.1016/0092-8674(91)90043-x.
We have isolated two Drosophila cDNA clones that rescue Saccharomyces cerevisiae deficient in CLN functions. One of these clones is the Drosophila homolog of the cdc2 gene. The second encodes a distant and new member of the cyclin family of proteins, cyclin C. It is highly homologous (72% identity) to a human clone isolated in a similar screen. Yeast cells rescued by a plasmid constitutively expressing this Drosophila cyclin C are unusually small, consistent with an unregulated high level of G1 cyclin function. Sequence comparisons identified regions conserved among the more distantly related cyclins. Based on these conserved elements, we identified homology between cyclins and the ras oncogene.
我们分离出了两个能拯救缺乏CLN功能的酿酒酵母的果蝇cDNA克隆。其中一个克隆是cdc2基因的果蝇同源物。另一个克隆编码一种细胞周期蛋白家族中关系较远的新成员——细胞周期蛋白C。它与在类似筛选中分离出的一个人类克隆高度同源（同一性为72%）。用组成型表达这种果蝇细胞周期蛋白C的质粒拯救的酵母细胞异常小，这与G1细胞周期蛋白功能不受调控的高水平一致。序列比较确定了在关系较远的细胞周期蛋白中保守的区域。基于这些保守元件，我们确定了细胞周期蛋白与ras癌基因之间的同源性。
