Weiss D S, Batut J, Klose K E, Keener J, Kustu S
Department of Plant Pathology, University of California, Berkeley 94720.
Cell. 1991 Oct 4;67(1):155-67. doi: 10.1016/0092-8674(91)90579-n.
The NTRC protein of enteric bacteria is an enhancer-binding protein that activates transcription in response to limitation of combined nitrogen. NTRC activates transcription by catalyzing formation of open complexes by RNA polymerase (sigma 54 holoenzyme form) in an ATP-dependent reaction. To catalyze open complex formation, NTRC must be phosphorylated. We show that phosphorylated NTRC has an ATPase activity, and we present biochemical and genetic evidence that NTRC must hydrolyze ATP to catalyze open complex formation. It is likely that all activators of sigma 54 holoenzyme have an ATPase activity.
肠道细菌的NTRC蛋白是一种增强子结合蛋白，可响应联合氮的限制而激活转录。NTRC通过在ATP依赖性反应中催化RNA聚合酶（σ54全酶形式）形成开放复合物来激活转录。为了催化开放复合物的形成，NTRC必须被磷酸化。我们发现磷酸化的NTRC具有ATP酶活性，并且我们提供了生化和遗传学证据，证明NTRC必须水解ATP才能催化开放复合物的形成。σ54全酶的所有激活剂可能都具有ATP酶活性。
